Microscope for multiplexed antibody-based imaging

用于多重抗体成像的显微镜

• 批准号: 502158695
• 金额: --
• 依托单位: Universität Münster
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/502158695?language=en
• 关键词: Microscope; multiplexed; antibody; based; imaging

To date, studies at single-cell resolution including scRNA-seq and CyTOF have focused on profiling populations of cells dissociated from tissues, where the crucial spatial context and information is lost. However, recent advances in microscopy techniques promise to overcome these limitations; imaging approaches that exploit highly multiplexed microscopy promise to simultaneously profile the expression of tens or hundreds of proteins within single cells whose spatial location is preserved. The device applied for here is a fully automated, highly multiplexed imaging platform based on cyclic immunofluorescence microscopy. This technique enables to survey expression profiles of several dozens of antigens on the very same tissue section. It is primarily intended to allow researchers at the University of Münster in the research areas of immunology, oncology, neuropathology, and reproductive medicine to apply an image-based cell profiling method by integrating spatial context and multiplexed antigen resolution. With the power of this state-of-the-art microscopy technique, the aim is to enable the Imaging Network Münster, and in particular the Institute of Experimental Pathology, to generate quantitative and spatially resolved maps of protein expression at subcellular resolution across an entire tissue section.

迄今为止，包括 scRNA-seq 和 CyTOF 在内的单细胞分辨率研究主要集中于分析从组织中分离出来的细胞群，这些细胞中关键的空间背景和信息都丢失了。然而，显微镜技术的最新进展有望克服这些限制。利用高度多重显微镜的成像方法有望同时分析单个细胞内数十或数百种蛋白质的表达，这些蛋白质的空间位置被保留。这里申请的设备是基于循环免疫荧光显微镜的全自动、高度多重成像平台。该技术能够调查同一组织切片上数十种抗原的表达谱。它的主要目的是让明斯特大学免疫学、肿瘤学、神经病理学和生殖医学研究领域的研究人员能够通过整合空间背景和多重抗原分辨率来应用基于图像的细胞分析方法。借助这种最先进的显微镜技术，我们的目标是使明斯特成像网络，特别是实验病理学研究所能够在整个组织切片上以亚细胞分辨率生成定量和空间分辨的蛋白质表达图。