Creating a number of 3D microstructures with various shapes and co-culture cell combinations by Cell Origami

人体是由多种细胞组成的不同形状的组织所组成的，具有多种细胞组合和多种形状的三维共培养结构是体外模拟人体组织的关键。然而，在以前，仅使用一种技术难以创建具有多种形状的3D微结构。细胞折纸是一种可以直接从二维到三维创建微结构的技术。通过改变微板的设计，可以容易地改变3D微结构的形状。本研究的目的是通过一种技术，细胞折纸，创造出许多不同形状的三维共培养微结构。利用该技术，可以简单地生成大量的正四面体、立方体和正十二面体等各种形状的三维微结构。

Human body is composed by different shapes of tissues included various cells, 3D co-culture structures with multiform cell combinations and multi-shapes are crucial to mimic human tissues in vitro. However, it is difficult to create 3D microstructures with various shapes using only one technique in previous. Cell origami is a technique which can create microstructures from 2D to 3D directly. By changing the design of microplates, the shapes of 3D microstructures are possible to be changed easily. The purpose of this research is to create a number of 3D co-culture microstructures with various shapes by only one technique, cell origami. Using this technique, amount of 3D microstructures with various shapes, Regular tetrahedron, cube and Regular dodecahedron, were created simply.