Comprehensive cancer therapy targeted Nek2 and translational research

针对 Nek2 的综合癌症治疗和转化研究

• 批准号: 20249062
• 负责人: NIMURA Yuji
• 金额: $ 31.28万
• 依托单位: Aichi Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249062/
• 关键词: NeK2; 分子標的治療; トランスレーショナルリサーチ; Nek2

The combination treatment using Nek2 siRNA and S1 suppressed the tumor growth in colorectal cancer model, more efficiently than Nek2 siRNA. The combined treatment of Nek2 siRNA with CDDP was also more effective than the administration of Nek2 siRNA alone. The combination exerted additive growth inhibition. The transmission of Nek2 siRNA to blood was the highest at 15 min and was less than 0.2μM (0.04% of doses). Nek2 siRNA disappeared within 1 h after the administration.No abnormalities in blood tests were observed. The No Observed Adverse Effect Level (NOAEL：No Observed Adverse Effect Levels) was 3.7 (mg/kg). Based on the data, we decided the Maximum Recommended Starting Dose (MRSD) as 0.0285 (mg/kg).

使用Nek 2 siRNA和S1的组合治疗抑制结直肠癌模型中的肿瘤生长，比Nek 2 siRNA更有效。Nek 2 siRNA与CDDP的联合治疗也比单独施用Nek 2 siRNA更有效。该组合发挥累加生长抑制作用。Nek 2 siRNA向血液的传递在15 min时最高，低于0.2μM（剂量的0.04%）。Nek 2 siRNA在给药后1 h内消失，血液检查未见异常。无明显不良作用水平（NOAEL：无明显不良作用水平）为3.7（mg/kg）。根据这些数据，我们决定最大推荐起始剂量（MRSD）为0.0285（mg/kg）。

项目成果

Silencing of Tousled-like kinase 1 sensitizes cholangiocarcinoma cells to cisplatin-induced apoptosis

• DOI: 10.1016/j.canlet.2010.03.011
• 发表时间: 2010-10-01
• 期刊: CANCER LETTERS
• 影响因子: 9.7
• 作者: Takayama, Yuichi;Kokuryo, Toshio;Senga, Takeshi
• 通讯作者: Senga, Takeshi

The combination treatments using siRNA and CDDP are effective therapeutic options for cancers

使用 siRNA 和 CDDP 的联合治疗是癌症的有效治疗选择

• 发表时间: 2011
• 作者: Ueno T;Ohori Y;Ito J;Hoshikawa S;Yamamoto S;Nakamura K;Tanaka S;Akai M;Tobimatsu Y;Ogata T.;Toshio Kokuryo
• 通讯作者: Toshio Kokuryo

Nek2 siRNAの非臨床試験と臨床応用

Nek2 siRNA的非临床研究和临床应用

• 发表时间: 2012
• 作者: 國料俊男;横山幸浩;江畑智希;菅原 元;高橋 祐;伊神 剛;深谷昌秀;上原圭介;板津慶太;吉岡裕一郎;梛野正人
• 通讯作者: 梛野正人

Nek2 as an effective molecular target for cancer treatment

Nek2作为癌症治疗的有效分子靶点

• 发表时间: 2010
• 作者: Kobavashi M.;Yoshimoto M.;Kawai K.;et al;Kokuryo T
• 通讯作者: Kokuryo T

Novel combination treatment for colorectal cancer using Nek2 siRNA and cisplatin

• DOI: 10.1111/j.1349-7006.2010.01504.x
• 发表时间: 2010-05-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Suzuki, Kazushi;Kokuryo, Toshio;Hamaguchi, Michinari
• 通讯作者: Hamaguchi, Michinari