DEVELOPMENT OF A TIME-DEPENDENT LIQUID MICRO-JET SPECTROSCOPY FOR RADIATION-INDUCED ANORMALY IN MIMETIC BIO-CELL MOLECULES

开发用于模拟生物细胞分子中辐射诱发异常的时变液体微喷射光谱

• 批准号: 21241017
• 负责人: UKAI Masatoshi
• 金额: $ 15.39万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21241017/
• 关键词: DNA放射線損傷; 化学的損傷修復; シンクロトロン放射光電子分光; 電子エネルギー損失分光; 時間相関分光; 液体分子線; 超励起分子のダイナミクス; 水素結合ネットワーク

To investigate the radiation-induced damage on DNA and its self-assembled repair both as the alternative thermo-dynamical restoration processes of bio-cells, we have developed a novel spectroscopic method for damage in bio-molecules under mimetic cell condition by combining a liquid-micro-jet synchrotron-radiation photoelectron spectroscopy and a newly built high energy electron energy loss spectroscopy. To observed time-dependent appearance of anomalous molecular species following irradiation, a high resolution mass spectrometry has also been developed.

为了研究辐射对DNA的损伤及其自组装修复作为生物细胞的另一种热力学修复过程，我们将液体-微射流同步辐射光电子能谱和新建立的高能电子能量损失能谱相结合，建立了一种新的模拟细胞条件下生物分子损伤的光谱方法。为了观察辐照后异常分子种类随时间变化的现象，还开发了高分辨率质谱法。

项目成果

X-ray absorption spectrosco pic analysis of DNA damage induced by soft X-rays

软X射线诱导的DNA损伤的X射线吸收光谱图分析

• 发表时间: 2011
• 作者: K. Fujii and A. Yokoya

放射線によるゲノムDNA損傷の初期過程と生体修復

辐射引起的基因组DNA损伤和生物修复的初始过程

• 发表时间: 2012
• 作者: 中山実樹;西澤明人;千田美紀;千田俊哉;菓子野康浩;福田雅夫;酒井保藏;横谷明徳
• 通讯作者: 横谷明徳

Measurement of unpaired electron species in dry DNA thin films irradiated with synchrotron soft X-rays around oxygen and nitrogen K-edge studied by EPR

EPR 研究的氧和氮 K 边周围同步加速器软 X 射线照射的干燥 DNA 薄膜中不成对电子物种的测量

• 发表时间: 2010
• 作者: T. Oka;A. Yokoya;K. Fujii;Y. Fukuda;and M. Ukai
• 通讯作者: and M. Ukai

Yield of single-, double-strandbreaks and nucleobase lesions in fully hydratedplasmid DNA films irradiated with high-LETcharged particles

用高 LET 带电粒子照射的完全水合质粒 DNA 膜中单链、双链断裂和核碱基损伤的产量

• 发表时间: 2012
• 期刊: Yield of single-, double-strandbreaks and nucleobase lesions in fully hydratedplasmid DNA films irradiated with high-LETcharged particles
• 作者: T. Ushigome;N. Shikazono;K. Fujii;R. Watanabe;M. Suzuki;C. Tsuruoka;H. Tauchi;and A. Yokoya
• 通讯作者: and A. Yokoya

A novel technique using DNA denaturation to detect multiply induced single-strand breaks in a hydrated olasmid DNA molecule by X-ray and ^4He^<2+>

一种利用 DNA 变性通过 X 射线和 ^4He^<2 > 检测水合质粒 DNA 分子中多重诱导的单链断裂的新技术

• 发表时间: 2011
• 期刊: Radiation Protection Dosimetry
• 影响因子: 1
• 作者: Kaneko;K.;A.Yokoya
• 通讯作者: A.Yokoya