Comprehensive Studies toward Synthesis of Glycoproteins

·Analysis of intracellular glycoprotein processing was conducted by using synthetic high-mannose-type glycans and their derivatives.·Thioester forming reaction and amino-protecting group were developed and applied to syntheses of glycoproteins and dendrimers.A novel efficient reaction which utilize an ultra-high pressure was developed。·Syntheses of glycoproteins which carry complex-type glycans were achieved.The most significant among them is the synthesis of erythmpoietin(EPO)，a medically important glycoprotein。体外assay clarified that its cell-proliferating activity was almost indistinguishable with native EPO.

・Analysis of intracellular glycoprotein processing was conducted by using synthetic high-mannose-type glycans and their derivatives.・Thioester forming reaction and amino-protecting group were developed and applied to syntheses of glycoproteins and dendrimers. A novel efficient reaction which utilize an ultra-high pressure was developed.・Syntheses of glycoproteins which carry complex-type glycans were achieved. The most significant among them is the synthesis of erythmpoietin (EPO), a medically important glycoprotein. In vitro assay clarified that its cell-proliferating activity was almost indistinguishable with native EPO.