Comprehensive Studies toward Synthesis of Glycoproteins

对内源性糖蛋白处理的分析是由使用合成高甘露糖型糖及其衍生物进行的。硫酯形成反应和氨基保护组已经开发和应用于糖蛋白质和皮肤修饰剂合成。一种新的有效反应，它利用了一种超高压的压力。glycoproteins的合成，即携带复合体型glycans已实现。它们中间最有意义的物质是红细胞素（EPO）的综合体，一种医学上重要的糖蛋白质。体外测定是明确的，因为它的细胞增殖活动几乎可以被原生EPO所独立。

・Analysis of intracellular glycoprotein processing was conducted by using synthetic high-mannose-type glycans and their derivatives.・Thioester forming reaction and amino-protecting group were developed and applied to syntheses of glycoproteins and dendrimers. A novel efficient reaction which utilize an ultra-high pressure was developed.・Syntheses of glycoproteins which carry complex-type glycans were achieved. The most significant among them is the synthesis of erythmpoietin (EPO), a medically important glycoprotein. In vitro assay clarified that its cell-proliferating activity was almost indistinguishable with native EPO.