Random and cooperative phenomena in multicomponent dynamic systems composed of motor proteins and cytoskeletal filaments (Stochastische und kooperative Phänomene in multikomponentigen dynamischen Synthesen bestehend aus Motormolekülen und Zytoskelettfilam

由运动蛋白和细胞骨架丝组成的多组分动态系统中的随机和协作现象

• 批准号: 5175400
• 负责人: Professor Dr. Erwin Frey
• 金额: --
• 依托单位: Lehrstuhl für Statistische und Biologische Physik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2003-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5175400?language=en
• 关键词: Random; cooperative; phenomena; multicomponent; dynamic

Our objective is to theoretically analyze random and cooperative phenomena in multicomponent dynamic systems composed of a "passive" filament, which is characterized by its structure and elasticity, and an "active" protein showing motor activity. Specifically we will study two systems: (a) binding of motor proteins to cytoskeletal filaments and (b) active networks composed of molecular motors and semiflexible polymers. (a) The equilibrium binding stoichiometry of dimeric proteins on lattices as a function of solution concentration and parameters characterizing the specific type of motor protein will be determined. We will further investigate irreversible binding without dissociation and the relaxational behavior towards equilibrium. (b) The material properties of active networks will be determined using methods from statistical mechanics and non-equilibrium physics. In particular we will calculate the dynamic structure factor. We will closely cooperate with Dr. R. Merkel, who studies active actomyosin networks via DLS experimentally.

我们的目标是从理论上分析随机和合作的多组分动态系统中的现象组成的“被动”的细丝，其特征在于它的结构和弹性，和一个“主动”的蛋白质显示电机活动。具体来说，我们将研究两个系统：（a）马达蛋白结合细胞骨架丝和（B）积极的网络组成的分子马达和半柔性聚合物。(a)将确定作为溶液浓度和表征特定类型马达蛋白的参数的函数的晶格上的二聚体蛋白的平衡结合化学计量。我们将进一步研究不可逆结合无解离和弛豫行为的平衡。(b)有源网络的材料特性将使用统计力学和非平衡物理学的方法来确定。特别地，我们将计算动态结构因子。我们将与R博士密切合作。默克尔说，她通过DLS实验研究了活跃的肌动球蛋白网络。