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イネを用いた短日植物の開花時期決定遺伝子の単離

利用水稻分离决定短日照植物开花时间的基因

基本信息

批准号：
08740619
负责人：
井澤毅
金额：
$ 0.64万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

项目摘要

イネは代表的な短日植物であり、開花時期に影響を与えるさまざまな遺伝子座が同定されているが、いまだ遺伝子単離には到っていない。そこで、我々は、非自律性転移因子Dsと転移酵素をコードする35S-AcTPase導入形質転換イネを交配して得られた個体(F1)の自殖後代(F2)を用い、開花時期が早くなる個体を選抜し、その後代を用いた解析を行っている。開花が早い突然変異系統B-10-1-80は安定な表現型を示し、Dsを1コピーゲノム内に持っている(Ds80)。この系統を短日・長日条件下で栽培すると、短日条件下では開花に差は見られないが、長日条件下で約10日開花が早くなる。このことから、この系統の変異した遺伝子は、本来、その機能が短日条件において抑制されるため、短日条件下での開花に変化がないと考えられる。F2解析の結果、B-10-1系統は2つの遺伝子変異を持つと推定された。そのうち、ひとつは優性変異、もう一方は、劣性変異と考えられる。Ds80と表現型の連鎖を調べたところ、開花時期が野生型と同じF2個体にDs80をヘテロの持つ個体が複数存在するので、優性変異がDs80のタッグによる可能性は低いことが明らかになった。現在、もう一方の劣性変異とDs80の連鎖についてF3世代を用いて解析中である。突然変異系統C-2-3-241系統のF5世代を用い、開花時期を調べたところ、短日条件下で平均6日、長日条件下で平均12日早く開花するとの結果を得た。このことから、C-2-3系統の変異した遺伝子は本来、恒性的に開花を抑制する機能を持つ遺伝子と推定される。C-2-3-241はDsを1コピーのみゲノム内に持ち(Ds241)、現在、表現型とDs241の連鎖について解析を行っている。RI系統及びYACクローンによるマッピングの結果、Ds80は染色体6番、Ds241は染色体11番にそれぞれ座乗することが明らかになった。

The short-day plant cycle and blossom period represented by the doctor are the same as those in the constellation of the plant and the plant. Ds transfer factor, I transfer factor, non-automatic transfer factor, 35S-AcTPase transfer factor, automatic transfer factor, automatic transfer factor, self-transfer factor, non-automatic transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-autonomic transfer factor, self-transfer factor, non-automatic transfer factor, self-transfer factor, self-transfer factor, non-autonomic transfer factor, In the morning, suddenly check the system Bmur10-1-80 stability table display, Ds 1 emergency response in the atmosphere (Ds80). Under the short-day and long-day conditions, the plants were cultivated under the short-day and long-day conditions, the flowers blossomed differently under the short-day conditions, and the flowers blossomed for about 10 days in the long-day conditions. In the system, the short-day condition is used to suppress the weather, and the blossom in the short-day condition is used to improve the safety of the system. The results of F2 analysis and the presumption of failure were maintained in the Btel 10-1 system. Let's talk about sex, sex. There is an error in the Ds80 table link between the wild type and the F2 body in the same period of bloom, and there is an error in the number of copies of the same body as the F2, while the possibility of Ds80 failure is lower than that of the normal one. At present, the F3 generation is in the process of analyzing the disadvantages of the F3 generation. All of a sudden, the F5 generation of the short-day system C Mel 2-3-241 was in full bloom, and the average flowering time was 6 days under short-day conditions and 12-day blossoms on average under long-day conditions. The original and constant blossom suppression mechanism of the Cmur2-3 system is capable of maintaining the presumption of error. Cmur2-3-241