Fundamental study on molecular farming using mulberry plant

Mulberry plant，a woody feedcrop for the silkworm，is an attractive species for molecular framing since the plant have superior agronomical characteristics.Transgenic mulberry clone，if produced，may provide a farming system for the production of valuable biomolecules。To realize this target，it is essential to establish the methods for(1)plantlet regeneration from explant tissues and(2)gene delivery into target tissue。High frequency of shoot regeneration was achieved by2step-culture system consisting of seedling culture for obtaining leaf explants and leaf culture for inducing shoot organogenesis。A critical factor in both cultures was medium composition in which2，3，5-triiodobenzoic acid and cytokinin such as6-benzylaminopurin and thidiazuron was included.For efficient gene delivery into regenerable leaf tissue，various bombardment conditions were optimized using a particle inflow gun device：gold particle as microprojectile，bombardment frequency，preculture period and conditioning treatment of target tissue。When the optimal conditions were combined，200-300gene expressing-cells per cm i D12锡D1 tissue were detected.Based on these results，it is possible to generate transgenic mulberry clones。

Mulberry plant, a woody feedcrop for the silkworm, is an attractive species for molecular framing since the plant have superior agronomical characteristics. Transgenic mulberry clone, if produced, may provide a farming system for the production of valuable biomolecules. To realize this target, it is essential to establish the methods for (1) plantlet regeneration from explant tissues and (2) gene delivery into target tissue. High frequency of shoot regeneration was achieved by 2 step-culture system consisting of seedling culture for obtaining leaf explants and leaf culture for inducing shoot organogenesis. A critical factor in both cultures was medium composition in which 2, 3, 5-triiodobenzoic acid and cytokinin such as 6-benzylaminopurin and thidiazuron was included. For efficient gene delivery into regenerable leaf tissue, various bombardment conditions were optimized using a particle inflow gun device : gold particle as microprojectile, bombardment frequency, preculture period and conditioning treatment of target tissue. When the optimal conditions were combined, 200-300 gene expressing-cells per cmィイD12ィエD1 tissue were detected. Based on these results, it is possible to generate transgenic mulberry clones.