Analysis of cell death inducing gene in human malignant gliomas

人恶性胶质瘤细胞死亡诱导基因分析

• 批准号: 09470292
• 负责人: YAMASHITA Junkoh
• 金额: $ 4.03万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470292/
• 关键词: glioma; apoptosis,; Caspase-3; カスパーゼ3; Fas; アポトーシス; 神経膠芽腫; CPP32; PARP

Cysteine proteases of caspase family (interleukin-1 beta -converting enzyme) have been implicated as components of cell death pathway and have been reported to involved in Fas, chemotherapeutic agents, and radiation-induced apoptosis. In this study, I assessed the expression of Caspase-1, Caspase-2 and Caspase-3 in 11 cases of primary astrocytic tumors ( five anaplastic astrocytomas, and six glioblastomas) by reverse transcription (RT)-PCR, Western blot analysis, immunohistochemistry, and in situ casspase-3 activity assay. The frequency of Caspase-1, Caspase-2 and Caspase-3 overexpression appears to correlate with the malignancy grade of astrocytic brain tumors. Furthermore, Caspase-2 and Caspase-3 overexpression and Caspase-3 activation may play an important role in the pathogenesis of necrosis, which is one of the histological hallmarks of glioblastoma.

半胱氨酸蛋白酶家族(IL-1β转换酶)被认为是细胞死亡途径的组成部分，并被报道参与Fas、化疗药物和辐射诱导的细胞凋亡。本研究采用逆转录聚合酶链式反应(RT-PCR)、Western印迹分析、免疫组织化学和原位caspase-3活性检测方法，检测了11例星形细胞肿瘤(间变性星形细胞瘤5例，胶质母细胞瘤6例)中Caspase-1、Caspase-2和Caspase-3的表达。Caspase-1、Caspase-2和Caspase-3过表达的频率与脑星形细胞肿瘤的恶性程度有关。此外，Caspase-2和Caspase-3的过度表达和Caspase-3的激活可能在肿瘤坏死的发病机制中起重要作用，而坏死是胶质母细胞瘤的组织学标志之一。

项目成果

新井政幸: "ヒト神経膠腫におけるカスパーゼ1、カスパーゼ2、カスパーゼ3の発現" 十全医会誌. 107. 87-94 (1998)

Masayuki Arai：“人神经胶质瘤中 caspase 1、caspase 2 和 caspase 3 的表达”十善医学会杂志 107. 87-94 (1998)。

Yamashita J,et al.: "Induction of various blood-brain barrier properties in non-neural endothelial cells by close apposition to co-cultured astrocytes." J Neuro-Oncol. 35. S49 (1997)

Yamashita J 等人：“通过与共培养的星形胶质细胞紧密结合，在非神经内皮细胞中诱导各种血脑屏障特性。”

O.Tachibana,et al: "Overexpression of ICE,CPP32 and ICH1 during the progression of human astrocytomas." J Neuro Oncol. 35. S43 (1997)

O.Tachibana 等人：“人类星形细胞瘤进展过程中 ICE、CPP32 和 ICH1 的过度表达。”

Yamashita J,Hayashi H,Yamamoto Y: "Induction of various blood-brain barrier properties in non-neural endothelial cells by close apposition to co-cultured astrocytes." J Neuro-Oncol. 35. S49 (1997)

Yamashita J、Hayashi H、Yamamoto Y：“通过与共培养的星形胶质细胞紧密结合来诱导非神经内皮细胞的各种血脑屏障特性。”

毛利正直: "グリオーマにおける多剤耐性関連蛋白の発現" 十全医会誌. 107. 33-43 (1998)

Masanao Mori：“神经胶质瘤中多药耐药性相关蛋白的表达”十善医学会杂志 107. 33-43 (1998)。