Development of the sensor for the determination of Na channel blockers and its application to the determination of fish and shellfish toxins and the survey of the physiological active products

The sensor for the determination of NaイイD1+イエD1 channel blockers consisted of frogs bladder， NaイイD1+イエD1 electrode， flow cell， peristaltic pump and recorder。The basic conditions for the determination of the Na channel blockers were established as follows。Preparation of Na channel electrode ： The tip of NaイイD1 electrode was covered by the frogs bladder·brane sandwiched between 2 sheets of cellulose acetate·brane。该电极集成在一个流动细胞上，连接到一个永久泵和注入端口。用于确定通道阻塞器的一些条件：初始载体解决方案含有8%NaCl，并调整到pH 4.8在30℃时转移到流动细胞，在0.8毫升/分钟。一旦背景信号稳定， 50 μ TTX样本溶液被注入传感器系统，响应被测量。样本的准备：在100 ° C的温度下，从每1克的鲸鱼和贝壳鱼毒素中提取1克，含有0.1%的乙酸和羟基酸。适当地和浮游生物毒素也从过滤器纸张中提取，这些浮游生物在100 ° C时正确地在0.01 M Acetic Acic acid，20分钟。D1通道阻断器装有海鲜和中国医学的草药，在100℃下从10分钟内提取出1克干燥的海鲜，在10分钟内提取出0.1 N羟基氯酸，在90℃下在热水中重新冲洗20 - 30克干燥的草药后，在1小时内提取出1克干燥的草药。NaイD1+イD1 channel blockers contained in seaweed and herb of chinese medicine were also searched.From these results， It had become feasible to determine NaイD1+イD1 channel blockers rapidly， simply， high sensitively and continuously。

The sensor for the determination of NaィイD1+ィエD1 channel blockers consisted of frogs bladder, NaィイD1+ィエD1 electrode, flow cell, peristaltic pump and recorder. The basic conditions for the determination of the Na channel blockers were established as follows.Preparation of Na channel electrode : The tip of NaィイD1+ィエD1 electrode was covered by the frogs bladder membrane sandwiched between 2 sheets of cellulose acetate membrane. This electrode was integrated within a flow cell connected to a peristaltic pump and injection port.Some conditions for the determination of a channel blockers : The initial carrier solution contained 8% NaCl and adjusted to pH 4.8 was transferred into the flow cell with 0.8 ml/min at 30℃. Once the background signal had stabilized, 50 ul of TTX sample solution was injected into the sensor system and the response was measured.Preparation of sample : Toxins of swellfish and shellfish was extracted from each 1 g of them with 0.1% acetic acid and hydrochloric acid at 100℃ for 10 min, respectively and planktons toxin was also extracted from the filter paper which the plankton was corrected on with 0.01 M acetic acid at 100 ℃ for 20 min. NaィイD1+ィエD1 channel blockers contained into seaweed and herb of Chinese medicine was extracted from 1 g of dried seaweed with 0.1 N hydrochrolic acid at 100℃ for 10 min and 20 to 30 g of the dried herb after refluxing with hot water at 90℃ for 1 hr, respectively.Toxins of swellfish and plankton were determined under the conditions mentioned above. NaィイD1+ィエD1 channel blockers contained in seaweed and herb of chinese medicine were also searched.From these results, It had become feasible to determine NaィイD1+ィエD1 channel blockers rapidly, simply, high sensitively and continuously.