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Steric visualization of diseased organs of laboratory small animals by three-dimensionally reconstructing MRI method

三维重建MRI方法对实验室小动物病变器官的立体可视化

基本信息

批准号：
09556064
负责人：
KUWABARA Mikinori
金额：
$ 8.83万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09556064/
关键词：
MRI three-dimensional steric image laboratory small animal model diseases mouse lung tumor mouse liver tumor volume rendering contrast agent マウス肺腫癌マウス肝腫癌ポリュームレンダリング画像解析小動物疾患三次元画像ラットマウス断層画像非侵襲撮像法

项目摘要

Using multislice MT images of thoracic and abdominal organs in laboratory small animals, three-dimensional (3-d) images were constructed by superimposing them on the computer with the volume ray tracing software. Positive contrast agent was orally administrated into stomach and duodenum cavities just before MR.imaging. Two-dimensional transversal multislices of MR images from thoracic to abdominal regions were taken by an SIS300/183 MRI system with a magnetic field of 7.05 T under the proton-weighting condition (TR=2000ms, TE=20ms). The slice thickness was I mm and the multislice spin-echo method was employed. Each organs (stomach, liver, duodenum, spleen, kidney heart, born and spine) was pseudo-colored by the commercially available graphic software Adobe Photoshop. The 3-d images were then constructed by superimposing these colored-multislices on a UNIX computer by the volume ray tracing method . In rats and mice, the thoracic and abdominal organs were visualized as the pseudo-colored 3-d MR images. The transparent 3-d images were also constructed which made us possible to clearly distinguished individual organ from other organs with respect the position, size and shape. Furthermore, the pathological changes of tissues could be recognized by this 3-d MRI technique in BALB/c mice with liver tumors derived from colon-26 cell line and in A/J mice with lung tumors induced by urethan.

利用实验小动物胸部和腹部器官的多层MT图像，利用体射线追踪软件在计算机上叠加，构建三维（3d）图像。造影前口服胃、十二指肠腔内阳性对比剂。在质子加权条件下（TR=2000ms， TE=20ms），使用磁场为7.05 T的SIS300/183 MRI系统对胸部至腹部的二维横向多层MRI图像进行扫描。切片厚度为1 mm，采用多片自旋回波法。每个器官（胃、肝、十二指肠、脾、肾、心、出生和脊柱）用市售图形软件Adobe Photoshop进行伪着色。然后，通过体射线追踪方法将这些彩色多片叠加在UNIX计算机上构建三维图像。在大鼠和小鼠中，胸部和腹部器官被可视化为伪彩色三维MR图像。构建了透明的三维图像，使我们能够清楚地区分单个器官与其他器官的位置，大小和形状。此外，在结肠-26细胞系衍生的BALB/c小鼠肝脏肿瘤和脲聚糖诱导的A/J小鼠肺肿瘤中，该三维MRI技术可以识别组织的病理变化。