Development of micromachined biosensor system for biomedical application

微机械生物传感器系统的研制在人工血液透析系统的废液管上安装了一个尿液传感器，用于连续监测尿液。将尿素酶滴在ISFET上，并通过戊二醛交联。该传感器系统还配备在使用人造血液的血液透析系统的废液排放管中。实验结果表明，该传感系统可用于透析过程中尿素的监测。我们还进行了SPV装置的基础研究，这有助于构建集成的pH传感装置。BOD传感器作为一种典型的微生物传感器，与SPV装置构建。BOD传感器通常采用氧电极作为传感器。然而，我们成功地制作了一个生物传感器，测量值相关的BOD。在原则上，当样品溶液中添加时，微生物在SPV设备上产生酸性化合物，该设备测量它。此外，我们提出了一种新的方法，将各种生物材料的密集排列的传感器上一次构建集成的多功能生物传感系统。利用流体自组装技术将固定有酶的微加工颗粒随机排列在芯片上。作为自组装所需的短程力，我们使用了重力。将固定有酶的微球放入微室中，将生物材料固定在微球上，然后将微球悬浮液加入到芯片上，通过随机流控自组装进行有序化。采用固定化酶催化化学发光法对生物材料的固定化进行了评价。

Development of micromachined biosensor system for biomedical applicationA urine sensor at a waste drain of an artificial blood dialysis system was deveIoped for the continuous monitoring of urine. Urease was dropped on an ISFET, and cross-linked by glutaraldehyde. The sensor system was also equipped in the waste drain of the blood dialysis system using artificial blood. The results showed the usefulness of the sensing system for the urea monitoring during dialysis.We also performed the fundamental research of a SPV device, which is useful to construct an integrated pH-sensing device. BOD sensor as a typical microbial sensor was constructed with the SPV device. Usually BOD sensor employs an oxygen electrode as a transducer. However, we succeeded to fabricate a biosensor that measures values related to BOD.In principle, when a sample solution was added, microorganism on a SPV device produces acidic compounds, and the device measures it.Furthermore, we proposed a novel method to immobilize various biomaterials on densely arrayed transducers at once to construct integrated multifunctional biosensing system. Microfabricated particles immobilized with enzymes for biosensor application were randomly arranged on a chip by fluidic self-assembly method. As the short-range force required in self-assembly, we employed gravity. A bead immobilized with enzyme fell into microchamber, After the immobilization of biomaterial on the microparticle, the suspension of microparticles were added on the chip to be ordered by random fluidic self-assembly. The immobilization of biomaterial was evaluated by chemiluminescence method catalyzed by immobilized enzymes.