Development of micromachined biosensor system for biomedical application

用于生物医学应用的微机械生物传感器系统的开发研制了一种用于连续监测尿液的人工血液透析系统废水排水孔尿液传感器。将脲酶滴在ISFET上，用戊二醛交联。该传感器系统还被安装在使用人工血液的血液透析系统的废液排水孔中。结果表明该传感系统在透析过程中尿素监测的有效性。我们还进行了SPV器件的基础研究，为构建集成的ph传感器件提供了基础。采用SPV装置构建了BOD传感器作为典型的微生物传感器。通常BOD传感器采用氧电极作为换能器。然而，我们成功地制造了一种测量与BOD相关值的生物传感器。原则上，当加入样品溶液时，SPV装置上的微生物产生酸性化合物，该装置对其进行测量。此外，我们提出了一种将多种生物材料同时固定在密集排列的传感器上的新方法，以构建集成的多功能生物传感系统。采用流体自组装的方法，将酶固定的微颗粒随机排列在芯片上。作为自组装所需的短程力，我们采用了重力。将酶固定的微球放入微腔中，将生物材料固定在微球上后，将微球悬浮液添加到芯片上，通过随机流体自组装进行有序排列。采用固定化酶催化化学发光法评价生物材料的固定化效果。

Development of micromachined biosensor system for biomedical applicationA urine sensor at a waste drain of an artificial blood dialysis system was deveIoped for the continuous monitoring of urine. Urease was dropped on an ISFET, and cross-linked by glutaraldehyde. The sensor system was also equipped in the waste drain of the blood dialysis system using artificial blood. The results showed the usefulness of the sensing system for the urea monitoring during dialysis.We also performed the fundamental research of a SPV device, which is useful to construct an integrated pH-sensing device. BOD sensor as a typical microbial sensor was constructed with the SPV device. Usually BOD sensor employs an oxygen electrode as a transducer. However, we succeeded to fabricate a biosensor that measures values related to BOD.In principle, when a sample solution was added, microorganism on a SPV device produces acidic compounds, and the device measures it.Furthermore, we proposed a novel method to immobilize various biomaterials on densely arrayed transducers at once to construct integrated multifunctional biosensing system. Microfabricated particles immobilized with enzymes for biosensor application were randomly arranged on a chip by fluidic self-assembly method. As the short-range force required in self-assembly, we employed gravity. A bead immobilized with enzyme fell into microchamber, After the immobilization of biomaterial on the microparticle, the suspension of microparticles were added on the chip to be ordered by random fluidic self-assembly. The immobilization of biomaterial was evaluated by chemiluminescence method catalyzed by immobilized enzymes.