Architecture and functional significance of nuclear laminae of different molecular composition

不同分子组成的核层的结构和功能意义

• 批准号: 5183722
• 负责人: Professor Dr. Reimer Stick
• 金额: --
• 依托单位: Arbeitsgruppe Zellbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5183722?language=en
• 关键词: Architecture; functional; significance; nuclear; laminae

The nuclear lamina is a main constituent of the nuclear skeleton. Its structure is cell type specific and its molecular composition is developmentally regulated. However, the functional significance of the differences in its molecular architecture is not clear. We will use ectopic expression of particular lamin proteins or lamin mutants in amphibian oocytes to experimentally alter the composition of the lamina in vivo. This strategy will allow us to analyze the molecular interactions within the lamina structure itself as well as with other components of the nuclear envelope and the nuclear interior. These experiments are aimed to give experimental support for the role of the lamina in maintaining the integrity and mechanical strength of the nuclear envelope and the nucleus and its potential role in establishing/maintaining the spatial pattern of the nuclear pore complexes. The analyses will serve as a first step to understand the function of more complex lamina structures in other biological systems, that are - for technical reasons - not or only hardly accessible to a detailed molecular and structural analysis.

核板是核骨架的主要组成部分。它的结构是细胞类型特有的，其分子组成是发育调节的。然而，其分子结构差异的功能意义尚不清楚。我们将使用在两栖类卵母细胞中异位表达特定的Lamin蛋白或Lamin突变体来实验改变体内的Lamin的组成。这一策略将使我们能够分析板层结构本身以及与核膜和核内部的其他成分之间的分子相互作用。这些实验的目的是为薄层在维持核膜和核的完整性和机械强度方面的作用以及它在建立/维持核孔复合体的空间格局中的潜在作用提供实验支持。这些分析将作为了解更复杂的片层结构在其他生物系统中的功能的第一步，由于技术原因，这些片层结构无法或仅难以进行详细的分子和结构分析。