Isolation and genetical analyses on phytase producing bacteria in the rumen

一系列的实验是在rumen中进行评价的phytin phosphohydrolysis活动和到孤立的phytase positive rumen bacteria。细菌蛋白酶活性被认为不是很高的。没有一个蛋白酶阳性细菌在使用的介质条件下孤立，而不是反应性的。两个细菌殖民地展示了清晰的区域，在PSM平台上孵化了在航空条件下。一种细菌展出了一种用于制造大光环的粉红色橡树群落，另一种制造相对较小光环的黄色橡树群落。在Rumen Inreased中，正性细菌的数目从接近不可重复水平到1.7 x10的D15的D1cfu/ml 1.0h后在早晨喂养后，在7.0h后在早晨喂养后在初始水平下降。KPU 001是一种石墨负的非动力直线杆，并且可以在模拟和有氧条件下生长。根据生理学和形态学结果，KPU 001认为属于Klebsiella，而且很可能属于K. pneumoniae。KPU 002建议对KPU 001 （DDBJ登记号：AB 012208）中的16 S rDNA基因组胶原蛋白质分析进行属于KPU 001 （DDBJ登记号：AB 012208）与肺炎克雷伯氏菌JCM 1662和KPU 002 （DDBJ登记号：AB 012207）与幽门杆菌无关。大麻中产生微生物的存在被质疑了。

A series of experiments were conducted to evaluate phytin phosphohydrolysis activity in the rumen and to isolate phytase positive rumen bacteria. Bacterial phytase activity was suggested not to be high. None of the phytase positive bacteria was isolated under anaerobic condition irrespective of the medium used. Two bacterial colonies showing clear zones were seen on PSM plates incubated under aerobic condition. One bacterium exhibited pinkish opaque colonies that made larger halo and the other yellowish colonies making relatively small halo. The numbers of phytase-positive bacteria in the rumen increased from nearly undetectable level to 1.7x10ィイD15ィエD1 cfu/ml 1.0h after morning feeding, thereafter declined to the initial levels at 7.0h after morning feeding. Kpu 001 was Gram negative non-motile straight rod and able to grow both under anaerobic and aerobic condition. According to the physiological and morphological results suggested Kpu 001 to belong the genus Klebsiella and most likely to be K.pneumoniae. Kpu 002 was suggested to belong to the genus Corynebactreium Phylogenetic analyses on 16S rDNA indicated that Kpu 001 (DDBJ Accession No : AB012208) was closely related to Klebsiella pneumoniae JCM 1662 and Kpu 002 (DDBJ Accession No. : AB012207 was to Corynebacterium vitarumen. The presence of potent phytase producing microorganisms in the rumen was questioned.