Mechanism of the angiogenesis in the collagen gel and clinical application to the transplantation

1)胶原凝胶血管生成过程培养2-3 d后，主动脉外植体成纤维细胞随机迁移，培养10 d后胶原凝胶中出现成管。2)整合素在血管生成中的作用位于主动脉外植体外周部分的迁移细胞中整合素α1和α2抗体阳性，α3整合素在靠近主动脉外植体的迁移细胞中明显表达。在毛细血管样管状结构周围的迁移细胞膜上存在α3相关的金颗粒。整合素α1和α2与细胞迁移有关，α3整合素对试管的形成有一定作用。3)生长因子在血管生成中的作用特别是在生长因子中，PDGF和bFGF促进了肌成纤维细胞的随机迁移。同时，TNF-α对这些细胞的随机迁移有明显的抑制作用。VEGF与其他生长因子不同，似乎是与血管管形成密切相关的因子。4)临床应用比目鱼肌的成纤维细胞迁移量比腓肠肌丰富。红肌如比目鱼肌，证实管状形成清晰。此外，我们还证实，即使是在芯层缺损中，用间胶原海绵也能发生血管生成。

1) Process of the angiogenesis in the collagen gelThere was the random migration of fibroblastic cells from the explant of aorta after 2-3 days incubation, and the tube-formation was occurred in collagen gel after 10 days incubation.2) The involvement of integrin in the angiogenesisThe migrating cells located in the peripheral part from the aortic explant were positive for integrins α1 and α2 antibodies α3 integrin was clearly expressed in the migrating cells located near the aortic explant. Gold particles associated with α3 were present on the membrane of the migrating cells surrounding capillary-like tube structure. Integrins α1 and α2 was related to the cell-migration and α3 integrin had some role for the tube formation.3)The involvement of the growth factor in the angiogenesisEspecially within the growth factors, PDGF and bFGF promoted random migration of myofibroblastic cells. In the meantime, TNF-α was committed inhibitory for the random migration of these cells. VEGF was different from other growth factor, and it seemed to be the factor which is closely related to the tube-formation.4) On the clinical applicationThe fibroblastic cell migration from soleus muscle is richer than that of the gastrocnemius. Of the red muscle such as soleus muscle, it was confirmed that the tube-formation was clear. In addition, it was confirmed that the angiogenesis occurred even in by the atelocollagen sponge for the corium defect.