权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanism of the angiogenesis in the collagen gel and clinical application to the transplantation

胶原凝胶血管生成机制及其在移植中的临床应用

基本信息

批准号：
09670025
负责人：
AKITA Masumi
金额：
$ 0.96万
依托单位：
Saitama Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670025/
关键词：
Angioenesis Integrin Growth factor Collagen gel Culture Aorta Skeletal muscle Immunohistochemistry 電子顕微鏡

项目摘要

1) Process of the angiogenesis in the collagen gelThere was the random migration of fibroblastic cells from the explant of aorta after 2-3 days incubation, and the tube-formation was occurred in collagen gel after 10 days incubation.2) The involvement of integrin in the angiogenesisThe migrating cells located in the peripheral part from the aortic explant were positive for integrins α1 and α2 antibodies α3 integrin was clearly expressed in the migrating cells located near the aortic explant. Gold particles associated with α3 were present on the membrane of the migrating cells surrounding capillary-like tube structure. Integrins α1 and α2 was related to the cell-migration and α3 integrin had some role for the tube formation.3)The involvement of the growth factor in the angiogenesisEspecially within the growth factors, PDGF and bFGF promoted random migration of myofibroblastic cells. In the meantime, TNF-α was committed inhibitory for the random migration of these cells. VEGF was different from other growth factor, and it seemed to be the factor which is closely related to the tube-formation.4) On the clinical applicationThe fibroblastic cell migration from soleus muscle is richer than that of the gastrocnemius. Of the red muscle such as soleus muscle, it was confirmed that the tube-formation was clear. In addition, it was confirmed that the angiogenesis occurred even in by the atelocollagen sponge for the corium defect.

1)胶原凝胶中的血管生成过程在培养2-3天后，成纤维细胞从主动脉外植体中随机迁移，培养10天后胶原凝胶中出现了管状结构。整合素在血管生成中的作用：在主动脉移植物周围部分的移行细胞中，α1和α2抗体呈阳性，α3整合素抗体呈阳性，α3整合素抗体呈阳性。在位于主动脉外植体附近的迁移细胞中表达。与α3结合的金颗粒存在于迁移细胞的膜上，围绕着毛细血管样管结构。整合素α1和α2与细胞迁移有关，α3整合素对成肌纤维细胞的管形成有一定的作用。3）生长因子参与血管生成，尤其是PDGF和bFGF促进成肌纤维细胞的随机迁移。同时TNF-α对这些细胞的随机迁移具有抑制作用。4）在临床应用上比目鱼肌成纤维细胞迁移较腓肠肌丰富，而腓肠肌成纤维细胞迁移较腓肠肌丰富。在比目鱼肌等红色肌肉中，确认了管形成是清楚的。此外，证实了即使在用于真皮缺损的去端胶原海绵中也发生了血管生成。