Mechanism of the angiogenesis in the collagen gel and clinical application to the transplantation

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1) Process of the angiogenesis in the collagen gelThere was the random migration of fibroblastic cells from the explant of aorta after 2-3 days incubation, and the tube-formation was occurred in collagen gel after 10 days incubation.2) The involvement of integrin in the angiogenesisThe migrating cells located in the peripheral part from the aortic explant were positive for integrins α1 and α2 antibodies α3 integrin was clearly expressed in the migrating cells located near the aortic explant. Gold particles associated with α3 were present on the membrane of the migrating cells surrounding capillary-like tube structure. Integrins α1 and α2 was related to the cell-migration and α3 integrin had some role for the tube formation.3)The involvement of the growth factor in the angiogenesisEspecially within the growth factors, PDGF and bFGF promoted random migration of myofibroblastic cells. In the meantime, TNF-α was committed inhibitory for the random migration of these cells. VEGF was different from other growth factor, and it seemed to be the factor which is closely related to the tube-formation.4) On the clinical applicationThe fibroblastic cell migration from soleus muscle is richer than that of the gastrocnemius. Of the red muscle such as soleus muscle, it was confirmed that the tube-formation was clear. In addition, it was confirmed that the angiogenesis occurred even in by the atelocollagen sponge for the corium defect.