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The role of actin microfilament cytoskeleton on the cell adhesion of chick embryonic arterial endothelium

肌动蛋白微丝细胞骨架对鸡胚动脉内皮细胞粘附的作用

基本信息

批准号：
09670036
负责人：
JINGUJI Yoichi
金额：
$ 0.96万
依托单位：
Gunma prefectural College of Health Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

This project aimed to reveal the distribution pattern of actin microfilaments especially of the stress fibers in the developing chick embryonic arterial endothelium. The immunofluorescence and electron microscopy, including laser scanning confocal microscopy were used in this study.Arterial endothelial cells of chick embryos aged over 9 days of incubation have well developed stress fibers oriented parallel to the major axis of the cell.When 0.1 ml of 20 μg/ml cytochalasin D was injected into the arterial branch of chorio-allantoic membrane of 15 to 16 day-old chick embryos, the distribution of stress fibers decreased within one hour. This change was accompanied by the appearance of a distinct bundle of actin microfilaments at the cell border. These actin bundles may support the cell to cell adhesion at the cell periphery where the adrehens junction may present. Some of the remaining stress fibers have a periodic banding pattern with rhodamine-phalloidin staining. Similar sarcomere-like pattern was observed in the stress fibers of cultured bovine aortic endothelial cells recovered from cytochalasin D treatment.Results of these studies suggest that : 1 The expression of endothelial stress fibers is induced by a cell elongation during embryonic development. 2 The endothelial stress fibers may support cell adhesion between cells or cell to substrate. 3 The sarcomere-like arrangement of actin microfilaments appeared in the developing stress fibers may represent one of the structural foundations.

本研究旨在揭示鸡胚动脉内皮细胞中肌动蛋白微丝，特别是应力纤维的分布规律。用免疫荧光和电子显微镜，包括激光共聚焦显微镜，观察了9日龄以上鸡胚动脉内皮细胞的应力纤维，应力纤维与细胞长轴平行。将20 μg/ml细胞松弛素D注入15 ~ 16日龄鸡胚绒毛尿囊膜动脉分支0.1ml，应力纤维分布在1小时内减少。这种变化是伴随着一个独特的肌动蛋白微丝束在细胞边缘的外观。这些肌动蛋白束可以支持细胞与细胞在细胞周边的粘附，其中粘附细胞连接可能存在。一些剩余的应力纤维具有罗丹明鬼笔环肽染色的周期性带型。细胞松弛素D处理的牛主动脉内皮细胞的应力纤维中也观察到类似的肌节样结构。这些研究结果表明：1内皮应力纤维的表达是由胚胎发育过程中的细胞伸长诱导的。2内皮应力纤维可支持细胞与细胞之间或细胞与基质之间的粘附。3.肌动蛋白微丝的肌节样排列可能是应力纤维发育的结构基础之一。