The study of the role of 2 kinds of TNF receptors regulating the TNF-induced apoptosis in eosinophils.

凋亡是慢性碘化炎症观察到的慢性碘化炎症的一种重要方法。使用人类白血病细胞系，Eol-1，我们研究了干扰素-伽马（IFN-γ）对Fas-和肿瘤转化因子-α （TNF）诱导凋亡的影响。THN和反单克隆抗体（CH-11）诱导Eol-1细胞的凋亡。预处理与IFN-γ增强CH-11诱导性凋亡与Fas的上调调节。However，治疗市场上有效地抑制了TNF诱导的凋亡。在流动细胞学分析中，Eol-1表达了两种肿瘤坏死因子受体（TNFR 1， 2）的表达，而TNFR 2的表达，但在IFN-Γ治疗后，TNFR 1没有明显地进行了调整。TNF诱导的凋亡被TNFR 1刺激抗体（htr-9）模拟，被TNFR 1阻塞抗体（H398）逆转。也就是说，TNFR 1介导的细胞毒性信号不受IFN-γ预处理、阻断TNFR 2的影响（utr-1） ... More 消除了IFN-Γ的抑制作用。在整合中，TNF诱导的凋亡最好是TNFR 1介导的，而且IFN-γ在EOL-1细胞线中的反凋亡效应的结果。进一步，我们研究了IFN-γ处理的EOL-1细胞中TNFR 2的信号转移路径。我们通过西方印迹分析揭示了TNFR 2激活了NF-kappaB的刺激，最近，我们报告了Rho/ROCK信号对细胞功能的影响。Rho/Rock Signaling抑制了myosin磷酸酶，并保持了磷酸化的myosin很高。岩石抑制剂（Y-27632）放松了麝香玫瑰花光滑的麝香接触。Myosin是众所周知的，可以调节细胞的形态变化和迁移。因此，我们研究了ROCK抑制剂的影响（Y-27632），并发现了Y-27632抑制了没有抑制Cai-D1 ++ Yi-D1的活性氧的迁移或产生的e-D1。这些发现是为纸张准备的。Less（低）

Apoptosis is important for the resolution of chronic eosinophilic inflammation observed in bronchial asthma. Using a human myeloblastic leukemia cell line, EoL-1, we investigated the effect of interferon-gamma (IFN-γ) on Fas- and tumor becrosis factor-α (TNF)-induced apoptosis. Both THN and anti-Fas monoclonal antibody (CH-11) induced apoptosis of EoL-1 cells. Pretreatment with IFN-γ enhanced the CH-11-induced apoptosis with up-regulation of Fas. However, the treatment markedly inhibited the TNF-induced apoptosis. In flow cytometric analysis, EoL-1 expressed two types of tumor necrosis factor receptors (TNFR1, 2) and the expression of TNFR2 but not of TNFR1 was up-regulated significantly after the IFN-Γ treatment. The TNF-induced apoptosis was mimicked by a TNFR1 stimulating antibody (htr-9), and was reversed by a TNFR1 blocking antibody (H398). Although the TNFR1-mediated cytotoxic signal was not affected by IFN-γ pretreatment, blocking TNFR2 by a specific antagonistic antibody (utr-1 … More ) canceled the inhibitory effect of IFN-Γ. In conclusion, TNF-induced apoptosis was mediated preferentially by TNFR1, and the anti-apoptotic effect of IFN-γ result from up-regulated TNFR2 in EoL-1 cell line. Further, we investigated the signal transduction pathway from TNFR2 in IFN-γ treated EoL-1 cells. We revealed that the stimulation through TNFR2 activated the NF-kappaB by western blot analysis.Recently, we reported that the effect of Rho/ROCK signaling on cell functions. Rho/Rock signaling inhibited the myosin phosphatase and kept the phosphoryrated myosin high. ROCK inhibitor (Y-27632) relaxed the muskarinic bronchiai smooth muscle contraction. Myosin is known to regulates the morphological change and migration of cells. So, we investigated the effect of ROCK inhibitor (Y-27632) on migration of eosinophols and found that Y-27632 inhibitted the migration of eosinophils without inhibitting the CaィイD1++ィエD1 influx or production of active oxygen. These findings were preparing for paper. Less