Detection of HGV mutation and pathological relation

为了解庚型肝炎病毒(HGV)的传播途径及其对肝功能的影响，我们调查了一般人群、血液透析患者、暗娼和丙型肝炎病毒(HCV)所致慢性肝病患者中HGV感染情况。我们研制了一种检测庚型肝炎病毒(HGV)的引物。用聚合酶链式反应检测HGV RNA，用ELISA法检测HGV包膜-2抗体(抗-E2)。298名普通人群中HGV-RNA和/或抗-E2的感染率为10.7%，225例血液透析患者中HGV RNA和/或抗-E2的感染率为10.7%，暗娼中HGV RNA和/或抗-E2的感染率为24.8%。慢性病毒血症发生率(21.7%)明显低于丙型肝炎病毒(83.2%)(p&lt；0.001)，显著高于乙肝病毒(5.3%)(p&lt；0.001)。在血液透析患者中，EGV RNA的患病率与输血次数和透析时间有关。在暗娼中，HGV和/或抗-E2的流行与从事卖淫的年限有关。274例慢性丙型肝病患者HGV-RNA阳性率为8.9%，HGV阳性与否与肝病严重程度无关。结论：HGV与丙型肝炎病毒和乙肝病毒一样，通过输血、医疗干预和性交传播，HGV感染对丙型肝炎病毒感染无明显影响。

To clarify the hepatitis G virus (HGV) transmission routes and its affect on liver function, we surveyed for HGV infection among the general population, hemodialysis patients, female prostitutes and patients with chronic liver disease caused by hepatitis C virus (HCV). We developed a primer for the detection of hepatitis G virus (HGV). We detected HGV RNA by PCR and measured antibody to envelope-2 of HGV (anti-E2) by ELISA.The prevalence of HGV RNA and/or anti-E2 was 10.7% in 298 persons of the general population, 10.7% in 225 hemodialysis patients, and 24.8% in female prostitutes. The chronic viremia rate of HGV (21.7%) was significantly lower than that of HCV (83.2%) (p<0.001) and significantly higher than that of hepatitis B virus (HBV) (5.3%) (p<0.001). Among the hemodialysis patients, the prevalence of EGV RNA was correlated with the number of blood transfusions and the duration of dialysis. Among female prostitutes, the prevalences of HGV and/or anti-E2 was correlated with number of years engaged in prostitution. The prevalence of HGV RNA in 274 patients with chronic liver disease C was 8.9%, with no relation between HGV presence or absence and the severity of liver disease. Moreover, interferon eliminates HGV RNA from the serum.In conclusion, HGV is transmitted by blood transfusion, medical intervention and sexual intercourse as are HCV and HBV.HGV infection has no apparent affect HCV infection.