Role of ATBF1 gene in carcinogenesis and progression in breast cancer

应用20多条染色体上的40个微卫星标记分析了乳腺癌的杂合性丢失（洛）。在乳腺浸润性导管癌中洛以17 p（44%）、16 q（31%）、17 q（29%）和13 q（23%）多见。洛缺失在个体患者中的累积与高组织学分级和显著不良预后密切相关。这表明洛缺失的积累发生在乳腺癌进展的晚期，可能是乳腺癌的一个有用的预后标志物。此外，16 q的洛在导管原位癌（DCIS）中也是常见的。本研究采用RT-PCR方法检测了乳腺癌细胞系（MCF 7、T47 D、ZR 75 - 1、BT-20、MDAMB 231、SKBR 3）和临床乳腺癌病例中16q22.3-23.1区域ATBF 1基因mRNA的表达。然而，mRNA的表达水平在乳腺癌和正常乳腺组织中相当低。在正常肺组织和支气管组织中有较高表达，尤其是正常肺组织。原位杂交显示正常支气管细胞表达高水平的ATBF 1 mRNA信号。提示AIBF 1基因可能在正常肺组织的发生和生长中起重要作用。

Loss of heterozygosity (LOH) was analyzed in breast cancer using 40 microsatellite markers on over 20 chromosomes. LOH was frequently seen in 17p (44%), 16q (31%), 17q (29%) and 13q (23%) in invasive ductal carcinama of the breast. Accumulation of LOH in individual patient was well associated with high histological grade and a significantly poor prognosis. This shows that accumulation of LOH occurs at a late stage of breast cancer progression and may be a useful prognostic marker of breast cancer. Furthermore, LOH in 16q was also frequently seen in ductal carcinoma in situ (DCIS). This shows that this chromosomal region may be important in early stage of carcinogenesis in DCIS.We investigated on the mRNA expression of ATBF1 gene located in 16q22.3-23.1 using RT-PCR method in breast cancer cell lines (MCF7, T47D, ZR75- 1, BT-20, MDAMB231, SKBR3) and clinical breast cancer cases. However, the level of mRNA expression was quite low in breast cancer and normal breast tissues. The expression was highly seen in normal lung and bronchial tissues, especially normal tissues. In situ hybridization showed that normal bronchial cell expressed high levels of ATBF1 mRNA signals. These data shows that AIBF1 gene may play an important role of genesis and growth of normal lung tissues.