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The role of dopamine D2 receptor in the maintenance of pregnancy

多巴胺D2受体在维持妊娠中的作用

基本信息

批准号：
09671655
负责人：
FURUTA Itsuko
金额：
$ 1.41万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671655/
关键词：
Do amine D2 receptor Mamtenance for pregnancy Luteal function Dopamine agonist Dopamine

项目摘要

Luteinization hormone and prolactin secreted by pituitary play an important role in the maintenance of luteal function especially at early pregnancy. It is well known that dopamine agonist (dopamine D2 receptor stimulant) is able to inhibits prolactin release from the pituitary gland. To study the role of dopamine D2 receptor in the maintenance of luteal function and pregnancy, we investigated the effect of cabergoline (CAB), a long-acting dopaminergic agonist , on the steroidogenesis of cultured rat granulosa cells and porcine luteal cells. Also the expression of dopamine D2 receptor mRNA in the mouse ovary was examined by RT-PCR. CAB inhibited the FSH-stimulated production of progesterone by cultured rat granulosa cell at concentrations ranging from 10ィイD1-8ィエD1 to 10ィイD1-4ィエD1M. At high concentrations (10ィイD1-5ィエD1 and 10ィイD1-4ィエD1M), CAB inhibited the production of estradiol by cultured rat granulosa cell. The production of progesterone by cultured luteal cell prepared from porcine corpus luteum was also inhibited by CAB at concentrations ranging from 10ィイD1-8]ィエD1 to 10ィイD1-4ィエD1M in the both condition with or without LH.Ovary from control, under inducing (48 h after injection of PMSG), preovulatory (6h after injection of hCG), ovulatory (12h after injection of hCG), postovulatory(24 and 48 h after injection of hCG) mice was examined for the expression of dopamine D2 receptor mRNA. However, the message of dopamine D2 receptor was not detected in the ovary at any ovulatory cycle These results, that dopamine D2 receptor stimulant directly inhibits the steroidogenesis of granulosa cells and luteal cells in vitro, suggest that dopamine D2 receptor may participate in the maintenance of luteal function and pregnancy.

垂体分泌的黄体化激素和催乳素在维持黄体功能中起重要作用，尤其是在妊娠早期。众所周知，多巴胺激动剂（多巴胺D2受体兴奋剂）能够抑制垂体分泌催乳素。为了研究多巴胺D2受体在维持黄体功能和妊娠中的作用，我们研究了卡麦角林（CAB），一种长效多巴胺能激动剂，对培养的大鼠颗粒细胞和猪黄体细胞的类固醇合成的影响。RT-PCR检测小鼠卵巢多巴胺D2受体mRNA的表达。CAB在10 μ mol/L D1-8 μ mol/L D1至10 μ mol/L D1-4 μ mol/L D1范围内抑制FSH刺激的大鼠颗粒细胞孕酮的产生。在高浓度（10 μ g/L D1-5 μ g/L D1和10 μ g/L D1-4 μ g/L D1 M）时，CAB抑制培养的大鼠颗粒细胞产生雌二醇。在有或无LH的条件下，CAB在10 μ mol/L D_（1 -8）~ 10 μ mol/L D_（1 -4）D_（1-M）范围内均能抑制猪黄体细胞孕酮的产生。（注射PMSG后48 h），排卵前（注射hCG后6小时），排卵在注射hCG后12 h，检测排卵后（注射hCG后24和48 h）小鼠多巴胺D2受体mRNA的表达。但在任何排卵周期的卵巢中均未检测到多巴胺D_2受体的信息。这些结果表明，多巴胺D_2受体激动剂直接抑制颗粒细胞和黄体细胞的类固醇合成，提示多巴胺D_2受体可能参与黄体功能和妊娠的维持。