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Involvement of cytoplasmic membrane-bound granulocyte neutral proteinases in mechanism of periodontal tissue destruction.

细胞质膜结合粒细胞中性蛋白酶参与牙周组织破坏机制。

基本信息

批准号：
09671900
负责人：
KUNIMATSU Kazushi
金额：
$ 1.92万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

To clarify possible roles of medullasin and cathepsin G, neutral proteinases in granulocytes, in periodontitis and drug-induced gingival overgrowth, we performed immunobiochemical and immunohistochemical studies using antibodies specific for each enzyme. Firstly, gingival crevicular fluid (GCF) was sampled from a severe or mild adult periodontitis patient (AP and YAP, respectively) and a juvenile periodontitis patient (LP) who all gave informed consent., followed by determination of medullasin level in GCE by enzyme immunoassay. The medullasin levels in GCOF from AP and LP were significantly higher than that from YAP.Secondly, we examined the distribution of medullasin and cathepsin G in periodontal tissues of adult periodontitis patients sampled during periodontal surgery. Each enzyme was largely found in the pocket epithelium and its adjacent connective tissue and showed mostly a degranulated profile. Moreover, there were a significant number of each enzyme-positive cells even in the … More deep connective tissues in the specimens of severely infiltrated inflammatory cells. However, these enzymes are mainly found in the cells are mainly outside the cells. Besides, these enzymes are suggested to be associated with the regulation of immune response and production of cytokines and growth peptides from host cells including fibroblasts. Recently, drug-induced gingival overgrowth is noted. Medullasin and cathepsin G have a possible role in the disease with unwanted side effect. Then we induced nifedipine-induced gingival overgrowth in rats by giving them a diet. containing the medication and examined the pathophysiological change of overgrowing gingival connective tissue for 32 weeks. There was a significant increase in the number of medullasin-bearing inflammatory cells in specimens of rats treated with nifedipine for 2 weeks. This tendency continued until the last. week of the experiment. The proportion of medullasin in the tissues increased up to 8 weeks with gingival overgrowth, indicating some association with its dlevelopment. These observations suggest that medullasin and cathepsin G are involved in periodontal diseases and play possible pathophysiological roles when they are localized close to the cytoplasmic membrane or bound to it. Less

为了澄清可能的作用，中性蛋白酶在粒细胞中，在牙周炎和药物诱导的牙龈增生，我们进行了免疫生物化学和免疫组织化学研究，使用抗体特异性的每种酶。首先，从一名重度或轻度成人牙周炎患者（分别为AP和雅普）和一名青少年牙周炎患者（LP）中采集龈沟液（GCF），所有患者均提供知情同意书。然后通过酶免疫测定法测定GCE中的庆大霉素水平。AP组和LP组GCOF中组织蛋白酶G的含量均显著高于雅普组。其次，我们检测了牙周炎患者牙周组织中组织蛋白酶G和组织蛋白酶G的分布。每种酶主要存在于囊袋上皮及其邻近结缔组织中，并且主要显示脱颗粒特征。此外，即使在组织中也存在显著数量的每种酶阳性细胞。 ...更多信息炎性细胞严重浸润标本中的深层结缔组织。然而，这些酶主要存在于细胞内，主要存在于细胞外。此外，这些酶被认为与免疫应答的调节以及从宿主细胞（包括成纤维细胞）产生细胞因子和生长肽有关。最近，药物引起的牙龈过度生长被注意到。髓蛋白酶和组织蛋白酶G可能在疾病中起作用，但有不良副作用。然后，我们诱导硝苯地平诱导牙龈增生大鼠给他们一个饮食。含药治疗32周，观察牙龈结缔组织增生的病理生理变化。硝苯地平治疗2周的大鼠标本中，具有炎性细胞数量显著增加。这种趋势一直持续到最后。实验的一周。随着牙龈过度生长，组织中的Pegasin的比例增加到8周，表明与其发育有关。这些观察结果表明，牙周炎和组织蛋白酶G参与牙周病，并发挥可能的病理生理作用时，他们被定位接近细胞质膜或绑定到它。