Involvement of cytoplasmic membrane-bound granulocyte neutral proteinases in mechanism of periodontal tissue destruction.

为了阐明粒细胞中的中性蛋白酶延髓蛋白和组织蛋白酶G在牙周炎和药物诱导的牙龈过度生长中的可能作用，我们使用针对每种酶的抗体进行了免疫生物化学和免疫组织化学研究。首先采集1例知情同意的成人重度牙周炎(AP)和轻度牙周炎(YAP)患者和1例青少年牙周炎(LP)患者的牙周沟液(GCF)，用酶免疫法测定牙周炎患者牙周炎患者牙周炎和牙周炎患者牙周炎患者牙周液中的髓质素水平。AP和LP患者牙周组织中的髓质素水平显著高于慢性牙周炎患者。其次，我们检测了牙周手术中成人牙周炎患者牙周组织中髓质素和组织蛋白酶G的分布情况。每种酶主要存在于袋上皮及其邻近的结缔组织中，主要表现为脱颗粒的轮廓。此外，即使在…中也有相当数量的酶阳性细胞炎性细胞浸润严重的标本中深部结缔组织较多。然而，这些酶主要存在于细胞内，主要存在于细胞外。此外，这些酶被认为与免疫反应的调节以及包括成纤维细胞在内的宿主细胞产生细胞因子和生长肽有关。最近，药物引起的牙龈过度生长被注意到。延髓素和组织蛋白酶G可能在这种具有不良副作用的疾病中起作用。然后，我们通过给大鼠饮食来诱导硝苯地平诱导的大鼠牙龈过度生长。灌胃给药，连续32周观察牙龈结缔组织过度生长的病理生理变化。硝苯地平治疗2周后，大鼠标本中含髓磷脂的炎性细胞数量明显增加。这一趋势一直持续到最后。实验的一周。随着牙周组织的过度生长，髓质素在组织中的比例增加到8周，这表明它与牙周发育有关。这些观察结果提示，髓质素和组织蛋白酶G与牙周疾病有关，当它们位于细胞膜附近或与细胞膜结合时，可能起到病理生理作用。较少

To clarify possible roles of medullasin and cathepsin G, neutral proteinases in granulocytes, in periodontitis and drug-induced gingival overgrowth, we performed immunobiochemical and immunohistochemical studies using antibodies specific for each enzyme. Firstly, gingival crevicular fluid (GCF) was sampled from a severe or mild adult periodontitis patient (AP and YAP, respectively) and a juvenile periodontitis patient (LP) who all gave informed consent., followed by determination of medullasin level in GCE by enzyme immunoassay. The medullasin levels in GCOF from AP and LP were significantly higher than that from YAP.Secondly, we examined the distribution of medullasin and cathepsin G in periodontal tissues of adult periodontitis patients sampled during periodontal surgery. Each enzyme was largely found in the pocket epithelium and its adjacent connective tissue and showed mostly a degranulated profile. Moreover, there were a significant number of each enzyme-positive cells even in the … More deep connective tissues in the specimens of severely infiltrated inflammatory cells. However, these enzymes are mainly found in the cells are mainly outside the cells. Besides, these enzymes are suggested to be associated with the regulation of immune response and production of cytokines and growth peptides from host cells including fibroblasts. Recently, drug-induced gingival overgrowth is noted. Medullasin and cathepsin G have a possible role in the disease with unwanted side effect. Then we induced nifedipine-induced gingival overgrowth in rats by giving them a diet. containing the medication and examined the pathophysiological change of overgrowing gingival connective tissue for 32 weeks. There was a significant increase in the number of medullasin-bearing inflammatory cells in specimens of rats treated with nifedipine for 2 weeks. This tendency continued until the last. week of the experiment. The proportion of medullasin in the tissues increased up to 8 weeks with gingival overgrowth, indicating some association with its dlevelopment. These observations suggest that medullasin and cathepsin G are involved in periodontal diseases and play possible pathophysiological roles when they are localized close to the cytoplasmic membrane or bound to it. Less