Molecular analysis of endoderm cell differentiation

内胚层细胞分化的分子分析

• 批准号: 10044204
• 负责人: TASAKA Masao
• 金额: $ 7.17万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044204/
• 关键词: Arabidopsis thaliana; SGR1; SCR; sgr mutants; endodermis; gravitropism; radial pattern formation; SGR7; SHR; scr変異株

We have identified the genes which related to the regulation of endoderm cell differentiation in Arabidopsis root hypocotyl and inflorescence stem. These genes are named SGR1/SCR(shoot gravitropism 1/scarecrow) and SGR7/SHR(Shoot gravitropism 7/short root). The SGR1/SHR have been cloned and it encodes a member of GRAS family. In this project, we made clear next 3 points.1.The shoot gravitropic mutants named sgr1 and sgr7 were allelic to scarecrow and shoot root respectively by genetic and molecular analysis. The expression pattern of SGR1/SCR gene was analyzed by in situ hybridization and expression pattern of marker gene using transgenic line containing SCR promoter :: GFP.2.SGR7/SHR was cloned. It was a member of same family of SGR1/SCR.3.The expression pattern of SGR1/SCR was distributed in a double mutant line of pin (pin formed) and sgr4 (shoot gravitropism 4). In this line, the endoderm cell layer in inflorescence stem was increased partially.

鉴定了拟南芥根、下胚轴和花序茎内胚层细胞分化调控的相关基因。这些基因分别被命名为SGR1/SCR（茎向地性1/稻草人）和SGR7/SHR（茎向地性7/短根）。SGR1/SHR已被克隆，它编码GRAS家族的一个成员。在这个项目中，我们明确了接下来的三点。突变体sgr1和sgr7分别与稻草人和茎根具有等位基因。利用含有SCR启动子的转基因系：：GFP.2，原位杂交分析SGR1/SCR基因的表达模式和标记基因的表达模式。克隆SGR7/SHR。它是SGR1/SCR.3同一家族的成员。SGR1/SCR的表达模式分布在引脚（引脚形成）和sgr4（茎向地性4）双突变系中。在这一细胞系中，花序茎的内胚层部分增加。

项目成果

Aida, M., et al.: "Shoot apical meristem and cotyledon formation during Arabidopsis embryogenesis : Interaction among the CUP-SHAPED COTYLEDON and SHOOT MERISTEMLESS genes"Development. 126. 1563-1570 (1999)

Aida, M., 等人：“拟南芥胚胎发生过程中芽顶端分生组织和子叶的形成：杯形子叶和芽分生组织基因之间的相互作用”的开发。

Ishida T.et al: "Involvement of CUP-SHAPED COTYLEDON genes in development of gynoecium and ovule in Arabidopsis thaliana"Plant Cell Phisiol.. 41. 60-67 (2000)

Ishida T.等：“拟南芥雌蕊和胚珠发育中杯形子叶基因的参与”Plant Cell Phisiol.. 41. 60-67 (2000)

Fukaki H.and Tasaka M: "Gravity perception and gravitropic response of infloral stems in Arabidopsis thaliana" Advance Space Research. in press. (1999)

Fukaki H.和 Tasaka M：“拟南芥花茎的重力感知和向重力响应”高级空间研究。

Tasaka, M., et al.: "The endodermis and shoot gravitropism"Trends in Plant Science. 4. 103-107 (1999)

Tasaka, M., et al.：“内皮层和茎向地性”植物科学趋势。

Lynn K., et al: "The PINHEA/ZWILLE gene acts pleiotropically in Arabidopsis devlopment and hasoverlapping functions with ARGONAUTE gene" Development. in press. (1999)

Lynn K. 等人：“PINHEA/ZWILLE 基因在拟南芥发育中发挥多效性，并且与 ARGONAUTE 基因具有重叠的功能”。