Nanopipettingrobot and Crystalisationhotel with UV-/VIS Camera

纳米移液机器人和配备 UV/VIS 相机的结晶酒店

• 批准号: 518584441
• 金额: --
• 依托单位: Heinrich-Heine-Universität Düsseldorf
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/518584441?language=en
• 关键词: Nanopipettingrobot; Crystalisationhotel; UV; VIS; Camera

We apply for a crystallization pipeline composed of a pipetting robot and a crystallization "hotel". The pipetting robot must be able to dispense the reservoirs in the 96-well plates as well as the nanoliter volume drops for the crystallization drops in a highly precise manner. The applied for crystallization hotel is used for the storage of the dispensed 96-well crystallization plates at a defined and stable temperature and is equipped with a UV-/VIS-camera/microscope which allows automated imaging of the plates as well as manually by the user. Both devices use the same software (“Rock Maker”) and use the same database, connecting them to a crystallization pipeline. Robot: The crystallization of proteins starts with the set-up of initial crystallization experiments. Commonly at least 1000 different crystallization drops with a tiny volume (100 - 200 nl) are set up for the first experiments. The drops are so small in volume to use small sample amounts since some proteins cannot be expressed and purified in large quantities (e. g. membrane proteins), or since some compounds (e. g. ligands, substrates, inhibitors etc.) are extremely expensive (for co-crystallization trials). Due to the fact that very small volumes of 100 nl has to be transferred the dispensing performance of the robot has to be extremely precise. This also leads to the need to have a closed system which is equipped with an adjustable humidifier to prevent these tiny drops from drying out. The robot must be able to handle all kinds of the commonly used technics (e. g. "sitting-drop", "hanging drop", "microbatch" und "Lipid Cubic Phase" (LCP)) and also the corresponding reservoirs of these 96-well plates. Hotel: The hotel must be equipped with a cooling system to enable a reliable and adjustable temperature for the storage of the crystallization plates. Furthermore, an integrated UV-/VIS-camera with microscope functionality and suited software is needed in order to ensure an automated imaging under these defined temperature conditions. Additionally, it is essential that this microscope can be use in a non-automatic manner so that the user can analyze the drops with individual settings. For the schedule of the automated imaging (documentation), the management of all data and the implementation of the relevant experimental parameters of each experiment, a database with a suited software is needed. This in turn means that the software of both, the pipetting robot and the storage hotel, have to be compatible with each other as well as with the used database to make this crystallization pipeline possible.

我们申请了一条由移液机器人和结晶“酒店”组成的结晶管道。移液机器人必须能够以高度精确的方式分配96孔板中的储液器以及用于结晶液滴的纳升体积液滴。申请的结晶库用于在规定的稳定温度下储存分配的96孔结晶板，并配备了UV/VIS相机/显微镜，可对板进行自动成像以及用户手动成像。这两种设备使用相同的软件（“Rock Maker”），并使用相同的数据库，将它们连接到结晶管道。机器人：蛋白质的结晶始于初始结晶实验的设置。通常为第一实验设置至少1000个具有微小体积（100 - 200 nl）的不同结晶液滴。液滴体积很小，使用少量样品，因为一些蛋白质不能大量表达和纯化（例如，G.膜蛋白），或者由于某些化合物（e. G.配体、底物、抑制剂等）非常昂贵（对于共结晶试验）。由于必须传输100 nl的非常小的体积，机器人的分配性能必须非常精确。这也导致需要有一个封闭的系统，该系统配备有可调节的加湿器，以防止这些微小的水滴变干。机器人必须能够处理各种常用的工艺（例如：G.“坐滴”、“悬滴”、“微批次”和“脂质立方相”（LCP））以及这些96孔板的相应储库。酒店：酒店必须配备冷却系统，以便为结晶板的存储提供可靠和可调的温度。此外，需要具有显微镜功能和合适软件的集成UV/VIS相机，以确保在这些定义的温度条件下自动成像。此外，该显微镜必须能够以非自动方式使用，以便用户能够通过单独设置分析液滴。对于自动成像（文件）的时间表，所有数据的管理和每个实验的相关实验参数的实施，需要一个带有合适软件的数据库。这反过来意味着移液机器人和存储酒店的软件必须相互兼容，并与所使用的数据库兼容，以使该结晶管道成为可能。