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Studies on CD4-2 positive cells a unique T cell subset of fish

CD4-2 阳性细胞（鱼类独特的 T 细胞亚群）的研究

基本信息

批准号：
22KJ1390
负责人：
Lau Lik Ming
金额：
$ 1.66万
依托单位：
Tokyo University of Marine Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2023
资助国家：
日本
起止时间：
2023-03-08 至 2024-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ1390/
关键词：
CD4-1 CD4-2 CD8α Ginbuna crucian carp Cell-mediated immunity

项目摘要

With the aid of monoclonal antibodies (mAbs) against CD4-1, CD4-2, and CD8α of ginbuna crucian carp (ginbuna), the roles of CD4-2 positive cells in the adaptive immunity of fish were investigated. In fiscal year (FY) 2022, the kinetics of CD4-1, CD4-2, and CD8α positive lymphocytes in ginbuna were studied through three kinds of in vivo experiments: (A) during cyprinid herpesvirus-2 (CyHV-2) infection; (B) under high-temperature water (HT) treatment after CyHV-2 infection; and (C) immunized with a live-attenuated vaccine against CyHV-2 infection. For experiment (A), I found that the number of CD4-1, CD4-2, and CD8α positive lymphocytes increased after CyHV-2 infection, indicating that CD4-2 positive lymphocytes play important roles against herpesvirus infection instead of Mycobacterial infection (reported in FY 2021) in fish. For experiment (B), I realized that the number of CD4-1, CD4-2, and CD8α positive lymphocytes increased rapidly after the secondary CyHV-2 challenge, indicating that the secondary immune responses require the help of both CD4-1 and CD4-2 positive lymphocytes in HT-treated fish. For experiment (C), after subsequent virulent virus challenge, I noticed that the number of CD4-2 positive lymphocytes significantly increased in ginbuna immunized with the live-attenuated vaccine whereas no significant difference was observed in CD4-1 positive lymphocytes. These results suggest that CD4-2 positive lymphocytes can have a different role from CD4-1 positive lymphocytes in fish.

利用抗银鲫CD 4 -1、CD 4 -2和CD 8 α单克隆抗体，研究了CD 4 -2阳性细胞在鱼类适应性免疫中的作用。在2022财政年度（FY），通过三种体内实验研究了银鱼中CD 4 -1、CD 4 -2和CD 8 α阳性淋巴细胞的动力学：（A）在鲤鱼疱疹病毒-2（CyHV-2）感染期间;（B）在CyHV-2感染后高温水（HT）处理下;以及（C）用针对CyHV-2感染的减毒活疫苗免疫。对于实验（A），我发现CyHV-2感染后CD 4 -1、CD 4 -2和CD 8 α阳性淋巴细胞的数量增加，表明CD 4 -2阳性淋巴细胞在鱼类中对抗疱疹病毒感染而不是分枝杆菌感染（2021财年报告）发挥重要作用。对于实验（B），我意识到在二次CyHV-2攻击后，CD 4 -1、CD 4 -2和CD 8 α阳性淋巴细胞的数量迅速增加，表明在HT处理的鱼中，二次免疫应答需要CD 4 -1和CD 4 -2阳性淋巴细胞的帮助。对于实验（C），在随后的强毒病毒攻击后，我注意到用减毒活疫苗免疫的银鱼中CD 4 -2阳性淋巴细胞的数量显著增加，而在CD 4 -1阳性淋巴细胞中没有观察到显著差异。这些结果表明，在鱼类中，CD 4 -2阳性淋巴细胞可以具有与CD 4 -1阳性淋巴细胞不同的作用。