Study on Physiological Function of Oxygenated Sterols in Blood

血液中含氧甾醇的生理功能研究

• 批准号: 59430025
• 负责人: IKEKAWA Nobuo
• 金额: $ 13.44万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59430025/
• 关键词: Oxygenated sterol; Gaschromatography-mass spectrometry; Micro-analysis; Blood component; Plant sterol; Plasminogen activator; 植物ステロール; 赤血球; 血液

This study deals with various biological effects of oxygenated sterols on blood components such as erythrocytes and platelets in relation with diseases like atherosclerosis and heart disease, and resulted in the following findings.(1) Sitosterol and fucosterol were found to produce plasminogen activator in cultured endothelial cells from bovine carotod artery.(2) Some of the oxygenated sterols found to cause rapid modulation of platelet aggregation in plasma. (22R)-22-Hydroxycholesterol found to specifically undertakes lysis of platelets and erythrocytes through its incorporation into membrane.(3) Cholesterol analogues with a carbene-generating substituent on the side chain were synthesized and their photochemical reactions were investigated in a model system.(4) Oxygenated sterols of fungus-infected silkworm carcass, Bombyx cum Botryte were analyzed.(5) 15-Oxygenated sterols were found to be a potent inhibitor of enzymatic conversion of dihydrolanosterol into cholesterol.(6) Natural sterols and terpenoids with biological activities related in this project were investigated and their structures were determined.(7) Serum sterols from patients (hypertension, atherosclerosis) were analyzed for oxygenated sterols.

本研究涉及含氧甾醇对血液成分如红细胞和血小板的各种生物学效应，与动脉粥样硬化和心脏病等疾病有关，并得出以下结果。(1)发现谷甾醇和岩藻甾醇在培养的牛颈动脉内皮细胞中产生纤溶酶原激活剂。(2)一些氧化固醇被发现能快速调节血浆中的血小板聚集。发现（22 R）-22-羟基胆固醇通过掺入膜特异性裂解血小板和红细胞。(3)合成了胆固醇类化合物，并在模型体系中研究了它们的光化学反应。(4)分析了真菌病家蚕尸体中的氧化甾醇。(5)发现15-氧化甾醇是二氢羊毛甾醇酶促转化为胆固醇的有效抑制剂。(6)对本项目涉及的具有生物活性的天然甾醇和萜类化合物进行了研究，并确定了其结构。(7)分析来自患者（高血压、动脉粥样硬化）的血清固醇中的氧化固醇。

项目成果

B.Ying, M.Morisaki, N.Ikekawa: "Oxygenated Sterol Deriatives. Their Identification from the Fungus-Infected Silkworm Carcass, Bombyx cum Botryte, and Their Effect on Growth and Sterol Metabolism of the Silkworm, Bombyx mori" Chem. Pharm. Bull. 32. 3003-30

B.Ying、M.Morisaki、N.Ikekawa：“含氧甾醇衍生物。它们从真菌感染的家蚕尸体（Bombyx cum Botryte）中进行鉴定，及其对家蚕 Bombyx mori 生长和甾醇代谢的影响”Chem。

H.Hagiwara;M.Shimooka;M.Morisaki;N.Ikekawa;Y.Inada: Thrombos.Res.33. 363-370 (1984)

H.Hagiwara；M.Shimooka；M.Morisaki；N.Ikekawa；Y.Inada：Thrombos.Res.33。

B.Ying;M,Morisaki;N.Ikekawa: Chem.Pharm.Bull.32. 3003-3008 (1984)

B.Ying；M，Morisaki；N.Ikekawa：Chem.Pharm.Bull.32。

Y.Saito;H.Shimada;T.Imada;T.Kikuchi;N.Ikekawa;Y.Inada: J.Membrane Biol.83. 187-191 (1985)

Y.Saito；H.Shimada；T.Imada；T.Kikuchi；N.Ikekawa；Y.Inada：J.Membrane Biol.83。

Y.Fujimoto, M.Kimura, F.A.M.Khalifa, N.Ikekawa: "Side Chain Structural Requirement for Utilization of Sterols by the Silkworm for Growth and Development. Non-stereoselective Utilization of the 24-Stereoisomeric Pairs of 24-Alkylsterols" Chem. Pharm. Bull.

Y.Fujimoto、M.Kimura、F.A.M.Khalifa、N.Ikekawa：“蚕生长和发育时利用甾醇的侧链结构要求。24-烷基甾醇的 24 立体异构对的非立体选择性利用” Chem。