Study of fetal physiology using intrauterine and extrauterine chronic preparation

使用宫内和宫外慢性准备研究胎儿生理学

• 批准号: 59440069
• 负责人: MASAHIKO MIZUNO
• 金额: $ 14.72万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59440069/
• 关键词: Fetal physilogy; Perinatal medicine; Extrauterine Fetal Incubation; Fetal electroencephalogram; 人工子宮; 胎外哺育; エクモ; 人工肺

Despite the recent progress of research methods, it is still difficult to analyze fetal behaviors and functions. in order to obtain more direct informations from a fetus, this new extrauterine incubation system has been developed by using 40 goat fetuses. The fetus is surrounded by artificial amniotic fluid and connected with the extracorporeal membrane oxygenator. The blood is drained from the umbilical arteries and returned to the umbilical vein. Several parameters are continuously recorded by the polygraph to monitor the fetal conditions. The fetuses could keep fairly physiological and stable conditions for a considerable period which ranged up to 10 days. It is considered that this system can be a useful method to investigate fetal pathophysiology. In fact, several researches have been undertaken to investigate the development of fetal central nerve system, the fetal behaviours, the fetal water metabolism and the fetal carbohydrats metabolism. It was elucidated by the analysis of fetal electroencephlogram that a fetus obtains the own biorhythm of the central nerve system around 120 days of fetal age.

尽管研究方法最近取得了进展，但分析胎儿的行为和功能仍然很困难。为了从胎儿身上获得更直接的信息，我们利用40个山羊胎儿开发了这种新型宫外孵化系统。胎儿被人工羊水包围，并与体外膜肺氧合器连接。血液从脐动脉排出并返回脐静脉。测谎仪连续记录几个参数以监测胎儿状况。胎儿可以在相当长的一段时间内（长达10天）保持相当的生理和稳定状态。人们认为该系统可以成为研究胎儿病理生理学的有用方法。事实上，已经进行了多项研究来调查胎儿中枢神经系统的发育、胎儿行为、胎儿水代谢和胎儿碳水化合物代谢。通过胎儿脑电图分析表明，胎儿在胎龄120天左右就获得了自己的中枢神经系统生物节律。

项目成果

岡井崇、桑公慶紀、今西由紀夫、上妻志郎、室之園悦雄、椋棒正昌、馬場一憲、施政庭、篠塚憲男、前田澤紀夫、秋葉和敏、海野信也、長屋憲、箕浦茂樹: 日本産科婦人科学会雑誌. 38ー8. 1209-1217 (1986)

Takashi Okai、Yoshiki Kuwako、Yukio Imanishi、Shiro Kozuma、Etsuo Muronozono、Masamasa Mukubo、Kazunori Baba、Seiseiwa、Norio Shinozuka、Norio Maedazawa、Kazutoshi Akiba、Shinya Unno、Ken Nagaya、Shigeki Minoura ：日本妇产科学会杂志38-8。1209-1217（1986）

岡井崇・桑原慶紀・今西由起夫・上妻志郎・室之園悦雄・椋棒正昌・馬場一憲・施政庭・篠塚憲男・前田津紀夫・秋葉和敬・海野信也・長屋憲・箕浦茂樹: 日本産科婦人科学会雑誌. 38ー8. 1209-1217 (1986)

Takashi Okai、Yoshiki Kuwabara、Yukio Imanishi、Shiro Kozuma、Etsuo Muronozono、Masamasa Mukubo、Kazunori Baba、Seiseiwa、Norio Shinozuka、Norio Maedazu、Kazutaka Akiba、Shinya Unno、Ken Nagaya、Shigeki Minoura：日本妇产科学会杂志38-8。1209-1217（1986）

国際産婦人科連合世界大会. (1985)

国际妇产科联合会世界大会（1985 年）。

上妻志郎、室之園悦雄、岡井崇、桑公慶紀、水野正彦: 日本産科婦人科学会雑誌. 38ー臨時増刊. S346 (1986)

Shiro Kozuma、Etsuo Muronozono、Takashi Okai、Yoshiki Kuwako、Masahiko Mizuno：日本妇产科学会杂志 38-特刊（1986 年）。

Yoshinori Kuwabara;Takashi Okai;Shiro Kozuma;Masahiko Mizuno et al.: Artificial Organs. 11ー3. 224-227 (1987)

Yoshinori Kuwabara；Takashi Okai；Shiro Kozuma；Masahiko Mizuno 等：人工器官。 11-3 224-227 (1987)