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Laser Spectroscopy in Biological Systems

生物系统中的激光光谱学

基本信息

批准号：
60300017
负责人：
KUSHIDA Takashi
金额：
$ 2.94万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60300017/
关键词：
laser spectroscopy bio-spectroscopy ヘマトポルフィリン誘導体

项目摘要

Laser spectroscopy has various advantages which are very desirable in the studies of biological systems; it hardly affects the object, abundant information can be obtained in real time, it is highly sensitive, the temporal and spatial resolutions are high, etc. However, the field of laser spectroscopy in biological systems has not been developed well yet.In order to accelerate research in this field, we organized a team consisting of fifteen investigators in the fields of laser engineering, optical spectroscopy, biophysics, medical science, and so on. Research meetings were held four times, in which 45 lectures such as "Basic Research on Cancers using Laser Spectroscopy" and "Picosecond Fluorescence Spectroscopy of DNA" were given, and active discussions were made basing on these lectures.The results of investigations performed by the members of the team are summarized in the pamphlet "Research Project Report". The main results contain the instantaneous measurements of cell-membrane potential distribution using a pulsed laser microscope, direct observation of the movements of actin-myosin molecules with a laser fluorescent microscope, picosecond spectroscopy in biological cells with the laser spot size of 0.8 <mu> m, and the development of an apparatus to measure the difference fluorescence spectrum and its application to the cancer detection.

激光光谱学在生物系统研究中具有对对象影响小、实时获取信息丰富、灵敏度高、时间和空间分辨率高等优点，但激光光谱学在生物系统中的研究还没有得到很好的发展，为了加速这一领域的研究，我们组建了一个由激光工程、光谱学、生物物理学、医学科学等领域的15名研究人员组成的团队。共举办了4次研究会，共举办了《激光光谱学癌症基础研究》、《DNA的皮秒荧光光谱学》等45场讲座，并在讲座的基础上进行了积极的讨论，团队成员的调查成果总结在《研究项目报告》小册子中。主要成果包括用脉冲激光显微镜瞬时测量细胞膜电位分布，用激光荧光显微镜直接观察肌动蛋白-肌球蛋白分子的运动，激光光斑大小为0.8微米的生物细胞的皮秒光谱，以及差示荧光光谱测量装置的研制及其在癌症检测中的应用。