Occuerrence and role of N- -alanyldopamine in the cuticle and pigments of insects

N-丙氨酰多胺在昆虫角质层和色素中的存在和作用

• 批准号: 60540457
• 负责人: UMEBACHI YOSHISHIGE
• 金额: $ 1.09万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60540457/
• 关键词: N<beta>-Alanyldopamine; <beta>-Alanine; Dopamine; Kynurenine; Papiliochrome; Papilio; Drosophila; 昆虫クチクラの硬化; ナミアゲハ; キアゲハ; クチクラ

The following five researches were carried out. (1) Level changes of <beta>-alanine, dopamine, and N-<beta>-alanyldopamine (NBAD) during the pupal stage of Papilio xuthus (Lepidoptera) were investigated. The accumulation and excretion of <beta>-alanine at the emergence of butterfly were also studied. Judging from both the present results and the results of previous papers, it is probable that both kynurenine and NBAD are incorporated from haemolymph into the wings or scales and there combine to each other to form the pale yellow pigment (Papiliochrome II). (2) Some chemical properties of the deep yellow pigment (Papiliochrome M) in the wings of Papilio machaon was studied. The results have clearly shown that the pigment is also composed of both kynurenine and NBAD. (3) The red pigment of the wings of Pachlioptera aristolochiae was confirmed to be a <beta>-alanine-containing quinone pigment. It is probable that the red pigment is an oxidationproduct of NBAD or N-<beta>-alanylnoradrenali … More ne by phenol oxidase. (4) Chemical properties of the puparial cuticle of yellow (y), black (b), and ebony (e) of Drosophila melanogater were investigated. The cuticle of y is yellowish brown and hard, and produces much more <beta>-alanine and a little less ketocatechol than in b and e. The protein content is lower than in b and e. On the other hand, the cuticles of b and e are white and plastic, and the <beta>-alanine content is only a little or zero, and the release of ketocatechol on acid hydrolysis is larger. The protein content is higher. For further study of chemical properties of these cuticles, 4% HCL-methanol extract has been confirmed to be suitable. And (5) the accumulation of insoluble-bound form of <beta>-alanine during the pupal stage of Papilio xuthus was investigated. Apart from the accumulation of soluble-bound form of <beta>-alanine (Papiliochrome II) shortly before emergence, the insoluble-bound form of <beta>-alanine accumulates in the body wall and wing-membrane of pharate adult at the late pupal stage when the body wall and wing-membrane are soft. This may represent the stage of pre-screlotization. Less

开展了以下五项研究。 (1)研究了凤蝶(鳞翅目)蛹期的β-丙氨酸、多巴胺和N-β-丙氨酰多胺(NBAD)的水平变化。还研究了蝴蝶羽化时β-丙氨酸的积累和排泄。从目前的结果和之前论文的结果来看，犬尿氨酸和 NBAD 很可能都是从血淋巴中掺入翅膀或鳞片中，并在那里相互结合形成淡黄色素（凤蝶色素 II）。 (2)研究了凤蝶翅膀中深黄色色素(Papiliochrome M)的一些化学性质。结果清楚地表明，该色素也是由犬尿氨酸和 NBAD 组成。 (3)确认马兜铃翅翅的红色素是含有β-丙氨酸的醌色素。红色素很可能是 NBAD 或 N-β-丙氨酰去甲肾上腺素被酚氧化酶氧化的产物。 (4)研究了黑腹果蝇黄色(y)、黑色(b)和乌木(e)蛹角质层的化学性质。 y的角质层呈黄棕色且坚硬，并且比b和e产生更多的β-丙氨酸和稍少的酮儿茶酚。蛋白质含量低于b和e。另一方面，b和e的角质层呈白色和可塑性，β-丙氨酸含量只有很少或为零，酸水解时酮儿茶酚的释放量较大。蛋白质含量较高。为了进一步研究这些角质层的化学性质，4% HCl-甲醇提取物已被证实是合适的。 (5)研究了Papilio xuthus蛹阶段不溶性结合形式的β-丙氨酸的积累。除了羽化前不久可溶性结合形式的β-丙氨酸（凤蝶色素II）的积累外，不溶性结合形式的β-丙氨酸在蛹期后期当体壁和翼膜柔软时在成虫的体壁和翼膜中积累。这可能代表了预硬化阶段。较少的

项目成果

Umebachi,Y.: Progress in Tryptophan and Serotonin Research. 1986. 405-408 (1987)

Umebachi,Y.：色氨酸和血清素研究进展。

Umebachi,Y.: Comp.Biochem.Physiol.82B. 763-766 (1985)

Umebachi,Y.：Comp.Biochem.Physiol.82B。

Umebachi, Y.: "Papiliochrome, a new pigment group of butterfly" Zool. Sci.2. 163-174 (1985)

Umebachi, Y.：“凤蝶色素，蝴蝶的一种新色素组”Zool。

Umebachi, Y.: "Accumulation and excretion of beta-alanine in Papilio xuthus (Lepidoptera: Papilionidae)" Comp. Biochem. Physiol.85B. 503-506 (1986)

Umebachi, Y.：“Papilio xuthus（鳞翅目：凤蝶科）中 β-丙氨酸的积累和排泄”比较。

Ishizaki,Y.: Comp.Biochem.Physiol.89B. (1988)

石崎，Y.：Comp.Biochem.Physiol.89B。