The neurotrophic or neurite-promoting effect of new compound, <beta> -citryl-L-glutamic acid on the primary cultured neuronal cells.

新化合物β-柠檬酰-L-谷氨酸对原代培养神经元细胞的神经营养或神经突促进作用。

• 批准号: 60570062
• 负责人: MIYAKE Masaharu
• 金额: $ 1.02万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570062/
• 关键词: Neurotrophic factor; β-シトリルグルタミン酸

1. The neurotrophic or neurite-promoting effect of <beta> -citryl-L-glutamic acid ( <beta> -CG) on the primary cultured neuronal cells from cerebrum, cerebellum, optic lobe or ciliary ganglion of chick embryo incubated for 7-10 days was examined. <beta> -CG had no effect on the peripheral neuronal cells from ciliary ganglion, but had the effect as survival factor on the central neuronal cells such as cerebrum, cerebellum and optic lobe, especially from optic lobe. The effect of <beta> -CG on the neuronal cells from optic lobe showed dose-responce curve between 20 and 200 <miero> M.2. The <beta> -CG content in the primary cultured neuronal cells from optic lobe was determined for 9 days. <beta> -CG increased extremely with the incubation time to day 6 and then decreased to day 9. The developmental change was similar to that of <beta> -CG concentration in the optic lobe during the growth of the chick embryo.3. The developmental change of <beta> -CG hydrolysing enzyme activity in the cerebrum and optic lobe was examined during the growth of chick embryo. The activity increased from incubation 9 to 20 in the optic lobe, but reached to the plateau level at incubation 14 in the cerebrum.<beta> -CG hydrolysing enzyme have been purified to the homogeneity from rat testes. This preparation had the molecular weight of 180 K and showed the nature as acidic glycoproteine and the affinity to DNA.

1. 研究了< β > -柠檬酸- l-谷氨酸（< β > -CG）对孵育7-10 d的鸡胚大脑、小脑、视叶或睫状神经节原代培养神经元细胞的神经营养或神经突促进作用。< β > -CG对睫状神经节外周神经元细胞无影响，但作为存活因子对大脑、小脑和视叶等中枢神经元细胞有影响，尤其是视叶。< β > -CG对视叶神经元细胞的影响在20 ~ 200 <miero> M.2之间呈剂量-反应曲线。测定原代培养的视叶神经元细胞< β > -CG含量9 d。< β > -CG随孵育时间的延长而急剧升高，至第9天逐渐降低。这种发育变化与鸡胚生长过程中视叶< β > -CG浓度变化相似。研究了鸡胚生长过程中大脑和视叶< β > -CG水解酶活性的发育变化。在第9 ~ 20个潜伏期，视叶的活动增加，而在第14个潜伏期，大脑的活动达到平台水平。< β > -CG水解酶已从大鼠睾丸中纯化到均匀性。该产物分子量为180k，具有酸性糖蛋白的性质和与DNA的亲和力。