Protection of infection using monoclonal antibody produced by Schistosoma japonicum cercariae attenuated by ultra violet-irradiation.

使用经紫外线照射减弱的日本血吸虫尾蚴产生的单克隆抗体来保护感染。

• 批准号: 60570171
• 负责人: TANAKA HIROSHI
• 金额: $ 1.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570171/
• 关键词: Schistosoma japonicum; ultra violet-attenuation; monoclonal antibody; 感染防御

BALB/c mice were immunized with cercariae of Schistosoma japonicum attenuated by irradiation with ultra violet ray at 254 nm. The highest protection attained was 47.3% by immunization with cercariae irradiated by UV at 500 mW sec/ <cm^2> . Mice were immunized with UV-attenuated cercariae and monoclonal antibodies (McAb) were produced by fusion of spleen cells from these mice. Twelve McAb, which recognized the surface of 24 hour-schistosomula by immunofluorescence, were selected and examined for reactivity to all stages of worms. Using these 12 McAb, antigenic similarity was examined between lung schistosomula and immature worms in the liver. Both were found to be close since the surface of both stages was recognized by 7 McAb and both were not by 1 McAb. The lung schistosomula, however, had a slightly different antigenicity because they were not recognized by 3 McAb which recognized immature worms in the liver. Protection of infection was examined in mice by injectng ascitic fluid of 4 McAb which recognized the surface of lung schistosomula, and 2 McAb showed comparatively high protection rates at 21.2 and 18.0%, respectively, and bound strongly to the tegument of adult and also to the parenchyma of adult worms and the egg. The killing effect of McAb on schistosomula was examined in vitro. They were damaged and killed by mouse neutrophils with the McAb, which bound to the surface of adult worms, supplemented with complement. Since these effective McAb recognized the common epitope distributed in the parenchyma and surface of adults, it was suggested that adult worms, in place of schistosomula, would be used as the source of material for obtaining antigens to induce the protective immunity.

用254 nm紫外线辐照减毒日本血吸虫尾蚴免疫BALB/c小鼠。用500毫瓦秒/ <cm^2>的紫外线照射尾蚴免疫，获得的最高保护率为47.3%。用uv减毒尾蚴免疫小鼠，通过融合小鼠脾细胞产生单克隆抗体（McAb）。选择12个免疫荧光法识别24小时血吸虫表面的单克隆抗体，检测其对各阶段血吸虫的反应性。利用这12个单克隆抗体，检测了肺血吸虫与肝脏未成熟血吸虫的抗原相似性。由于两个阶段的表面都能被7个单克隆抗体识别，而两个阶段都不能被1个单克隆抗体识别，因此发现两者是接近的。然而，肺血吸虫的抗原性略有不同，因为它们不能被识别肝脏中未成熟蠕虫的3mcab识别。在小鼠腹水中注射识别肺血吸虫表面的4种单克隆抗体，检测其对感染的保护作用，其中2种单克隆抗体的保护率较高，分别为21.2%和18.0%，并与成虫的被皮、成虫的薄壁和虫卵结合较强。体外研究了单抗对血吸虫的杀伤作用。在补充补体的情况下，与成虫表面结合的单克隆抗体被小鼠中性粒细胞破坏和杀死。由于这些有效的单克隆抗体识别了分布在成虫薄壁和表面的共同表位，因此建议将成虫代替血吸虫作为获得抗原的材料来源，以诱导保护性免疫。

项目成果

松田肇: 寄生虫学雑誌. 36. 36- (1987)

Hajime Matsuda：寄生虫学杂志 36. 36- (1987)。

SATOSHI NAKAMURA: "Immunizing effect of ultraviolet irradiated cercariae of Schistosoma japonicum in BALB/c mice." Japan. J. Parasitol.35. 148 (1986)

SATOSHI NAKAMURA：“紫外线照射的日本血吸虫尾蚴对 BALB/c 小鼠的免疫作用。”

中村哲: 寄生虫学雑誌. 35. 148- (1986)

中村聪：寄生虫学杂志 35. 148- (1986)

HAJIME MATSUDA: "Passive immunization of mice with monoclonal antibodies induced by immunization of ultraviolet-irradiated Schistosoma japonicum cercariae." Japan. J. Parasitol.36. 36 (1987)

HAJIME MATSUDA：“用紫外线照射的日本血吸虫尾蚴免疫诱导的单克隆抗体对小鼠进行被动免疫。”

松田肇: 寄生虫学雑誌. 35. 63- (1986)

Hajime Matsuda：寄生虫学杂志 35. 63- (1986)。