Macromolecular approaches for the preparation of artificial proteins

制备人工蛋白质的大分子方法

• 批准号: 5209184
• 负责人: Professor Dr. Harm-Anton Klok
• 金额: --
• 依托单位: Laboratoire des Polymères
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5209184?language=en
• 关键词: Macromolecular; approaches; preparation; artificial; proteins

Three different macromolecular approaches for the development of protein mimics are proposed. The first, non-covalent approach explores the self-assembly of peptide-based diblock copolymers. The self-assembly is driven by a combination of hydrophobic forces and specific interactions between the peptide blocks. The diblock copolymer molecules are designed to self-assemble into aggregates of well-defined size and in aqueous solution, and allow a covalent post-modification in order to fix the structure of the protein-mimics. The second approach involves the design and synthesis of non-linear polypeptide architectures. Specific examples that will be investigated include star-shaped and dendritic graft polypeptides. Such molecules already inherently possess a dense, globular shape, and do not require folding or other self-organization processes to adopt protein-like structures. The third approach focuses on the preparation and characterization of multidomain peptide copolymers that contain well-defined secondary structural elements. It is anticipated that the intramolecular association of these secondary structural elements will induce of the polypeptide into protein-like architectures.

提出了三种不同的大分子方法来开发蛋白质模拟物。第一，非共价的方法探索肽基二嵌段共聚物的自组装。自组装是由疏水力和肽块之间的特定相互作用的组合驱动的。二嵌段共聚物分子被设计成在水溶液中自组装成明确大小的聚集体，并允许共价后修饰，以固定蛋白质模拟物的结构。第二种方法涉及非线性多肽结构的设计和合成。将研究的具体例子包括星形和树突状嫁接多肽。这些分子本来就具有致密的球状结构，不需要折叠或其他自组织过程就能形成类似蛋白质的结构。第三种方法侧重于含有明确定义的二级结构元素的多域肽共聚物的制备和表征。预计这些二级结构元件的分子内结合将诱导多肽形成蛋白质样结构。