Analysis of immunological mechanisms for antibody production to hepatitis B surface antigen.

点击翻译按钮获取中文摘要

1. Enhancement of antibody production to hepatitis B surface antigen by anti-idiotypic antibody. Studies were undertaken to determine whether anti-idiotypic antibody against antibody to hepatitis B surface antigen (anti-HBS) could modulate in vitro anti-HBS production by human peripheral blood mononuclear cells stimulated with pokeweed mitogen. Peripheral blood mononuclear cells from patients positive for serum anti-HBs produced significantly increased amounts of anti-HBs by the addition of F <(ab')_2> fragments of anti-anti-HBs as well as purified HBsAg when compared to those in cultures with pokeweed mitogen alone, indicating that anti-idiotypic antibody modulates the immune response to HBsAg.2. In vitro synthesis of antibody to hepatitis B virus antigen by circulating lymphocytes from chronic HBsAg carriers and patients with acute hepatitis B. The synthesis of IgG, anti-HBs and anti-HBc was measured in pokeweed mitogen-stimulated cultures of peripheral blood mononuclear cells from chronic HBsAg carriers, patients with acute hepatitis B (HAB) and subjects with anti-HBs in serum (controls). Coculture experiments indicated that decreased B cell function and increased suppressor T cell function specific for HBsAg contributed to the lack of anti-HBs in serum of chronic HBsAg carriers and AHB patients during the recovery phase.3. Enhancement of antibody production to hepatitis B surface antigen by interleukin 2. We studied to assess whether induction of anti-HBs production by cultured lymphocytes which had been conducted with pokeweed mitogen could be replaced by interleukin 2 (IL2). In cultures stimulated with purified HBsAg and IL2, the comparable levels of anti-HBs production to those obtained in cultures stimulated with pokeweed mitogen alone occured when peripheral blood mononuclear cells from patients positive for serum anti-HBs and recipients of HBsAg vaccine were used as effector cells. However, IL2 alone did not induce anti-HBs production.