Physiology and Biochemistry of Fibronectin; Comprehensive Approaches from Basic to Clinical Studies.

本研究的目的是研究细胞黏附糖蛋白的基础和临床生理生化，特别是纤维连接。结果总结如下。1. 通过x射线暴露失活分析，估计纤维连接蛋白细胞附着位点的有效质量为90 KDa。2. 纤维连接蛋白在人胎盘的组织组织中起重要作用，因为胎盘会合成胎盘特异性纤维连接蛋白。3. 从纤维连接蛋白与癌基因的关系研究来看，一些抑制癌基因功能的抗生素既增加了纤维连接蛋白mRNA的量，又增加了纤维连接蛋白的合成。4. 发现人体血液中纤维连接蛋白的浓度与营养状况有关。5. 在类风湿性关节炎中，发现软骨表面的纤维连接蛋白在促进肌膜向关节表面延伸方面起重要作用。6. 在组织中，纤维连接蛋白和蛋白聚糖及其细胞表面受体的结合被发现可以调节细胞对底物的附着和扩散。7. 新鲜制备的角膜上皮细胞不表达纤维连接蛋白受体。它们在伤口愈合过程中改变表达受体。

The aim of this research project is to investigate basic and clinical physiology and biochemistry of cell-adhesive glycoproteins, especially fibronection. Results are summarized as follows. 1. An effective mass of a cell attachment site in fibronectin was estimated as 90 KDa from analyzing inactivation by X-ray exposure. 2. Fibronectin is suggested to play an important role in tissue organization of human placenta, because placenta synthesized placenta-specific fibroncctin. 3. From studies on relationship between fibronectin and oncogenes, some antibiotics suppressing oncogene function increased both amounts of fibronectin mRNA and fibronectin synthesis. 4. Concentration of fibronectin in human blood is found to relate to the nutritive conditions. 5. In rheumatoid arthritis, fibronectin on the cartilage surface is found to play an important role in promoting pannus extension onto the articular surface. 6. In tissue organization, the combination of fibronectin and proteoglycans together with their cell surface receptors is found to regulate attachment and spreading of cells to substrate. 7. Corneal epithelial cells prepared freshly did not express fibronectin rceeptor. They changed to express the receptor during wound healing.