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Studies on the Production of Tetrodotoxin and the Toxification of Pufferfish.

河豚毒素的产生及河豚毒性的研究。

基本信息

批准号：
61304025
负责人：
SHIMIZU Chiaki
金额：
$ 10.5万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

1. In this research, the tetrodotoxin (TTX) productive bacteria isolated from Jania sp., Octopus maculosus, Zosimus aeneus, Carcinoscorpius rotundicauda and pufferfishes are 19 strains of Vibrio naceae including 6 strains of V. alginolyticus sp., 8 strains of Pseudomonas alteromonas sp. including A. alga sp. nov. and A. tetrodonas sp. nov. and Sewanella putrefaciens sp., but the TTX productive capacity of these bacteria is decrease extremely when cultured in a culture medium or preserved for a long time. Therefore, the explanation of TTX bio-synthesis or toxification of pufferfish with these bacteria are task of extreme difficulty.2. New detective method of TTX in urine or plasma was discovered.3. TTX analogs identified from pufferfish are 6-epi TTX and 11-deoxy TTX, and from newt are 6-epi TTX, 11-deoxy TTX, anhydro-6-epi TTX, 11-deoxy-4-epi TTX and anhydro-11-deoxy TTX.4. When njected TTX and anhydro TTX mixture (1 : 4) to abdominal cavity of pufferfishes, the reciprocal conversion between both are very few. Accordingly, it is out of the bonds of possibility that anhydro TTX is the source of TTX in pufferfish.5. Nonpoisonous pufferfishes bred with poisonous pufferfihes in the sea water contained high percentage of antibiotics are toxify, so it is probable that a TTX productive bacteria is not the source of toxification in research.6. Toxicity of japanese newt is more than toxicity of poisonous pufferfish's liver.7. TTX is not need to spawning and growth in poisonous pufferfish.

1. 本研究分离的产河豚毒素（TTX）细菌为19株n弧菌科，其中6株为溶藻弧菌，8株为异交假单胞菌，包括A. alga sp.、A. tetrodonas sp.和Sewanella putrefaciens sp.；但这些细菌在培养基中培养或长期保存后，其TTX生产能力急剧下降。因此，解释TTX的生物合成或这些细菌对河豚的毒性是一项极其困难的任务。发现了尿、血浆中TTX检测的新方法。从河豚中鉴定的TTX类似物有6-epi TTX和11-脱氧TTX，从蝾螈中鉴定的TTX类似物有6-epi TTX、11-脱氧TTX、无羟基-6-epi TTX、11-脱氧-4-epi TTX和无羟基-11-脱氧TTX.4。当将TTX和无水TTX混合物（1:4）注射到河豚腹腔时，两者之间的倒数转换很少。因此，无水TTX是河豚体内TTX的来源是不可能的。无毒河豚与有毒河豚在海水中繁殖，其中含有高比例的抗生素是有毒的，因此在研究中，产生TTX的细菌很可能不是中毒的来源。日本蝾螈的毒性大于有毒河豚肝脏的毒性。有毒河豚不需要TTX来产卵和生长。