The effect of oxygen tension on the changes in the level of endogenous ABA corresponding the dormancy-breaking in rice seed.

氧张力对水稻种子休眠打破所对应的内源ABA水平变化的影响。

• 批准号: 61560018
• 负责人: HAYASHI Mitsuru
• 金额: $ 1.15万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560018/
• 关键词: Dormancy in rice seed; Inactivation of ABA; 酸素濃度; ABA; 稲種子の休眠現象; ABAの不活性化

The seeds of 3 varieties having the different dormancy periods were tested for germination under 100%, 20%, and 0% oxygen contents with interval of 10 days during the dormancy. The dormancy-breaking was promoted under the high oxygen tension but it was inhibited under the low oxygen tension. Both the degree of the promotion and of the inhibition were positively proportional to the intensities of the dormancy. The soaked seeds treated with high tenperature of 40゜C, for 2 days under the conditions of 20% and 0% oxygen contents were sown in the flasks containing 100%, 20%, and 0% oxygen, respectively. The dormancy of the seeds treated under the condition of 20% oxygen was broken most effectively at the high oxygen tension, but under the condition of 0% oxygen, dormancy-breaking was inhibited exceedingly. On the other hand, the dormancy of seeds treated under the condition of 0% oxygen was broken effectively at the high oxygen tension, but under the condition of 0% oxygen, no dormancy-breaking occurred at all. When artificial treatments such as husking or injury, were given to soaked or dry seeds, the lowering in the level of ABA occurred with both treatments, but the lowering was much more remarkable in soaked seeds than in dry seeds. The level of the ABA in the soaked seeds treated with high temperature was lowered remarkably under the condition of 100% oxygen, but it stayed unchangeably under the condition of 0% oxygen, And the fact that endogenous ABA content stayed unchangeably under the condition of 0% oxygen, was proved more clearly by the quantitative analysis using gas chromatography.

以3个休眠期不同的品种种子为材料，在休眠期内间隔10 d，分别在100%、20%和0%氧含量条件下进行发芽试验。高氧张力促进休眠的打破，而低氧张力则抑制休眠的打破。促进和抑制的程度都与休眠的强度成正比。将经40 ℃高温、20%和0%氧气条件下处理2天的种子分别播种在100%、20%和0%氧气的烧瓶中。20%氧处理的种子在高氧压条件下打破休眠效果最好，而在0%氧条件下，打破休眠受到极大的抑制。而在高氧压条件下，0%氧处理的种子能有效打破休眠，而在0%氧条件下，则不能打破休眠。当对浸泡或干燥种子进行人工处理时，两种处理都发生了阿坝水平的降低，但浸泡种子的降低比干燥种子更显著。100%氧气条件下，高温处理的种子中阿坝含量显著降低，而在0%氧气条件下，ABA含量基本保持不变。气相色谱定量分析进一步证明了内源阿坝含量在0%氧气条件下基本保持不变的事实。