权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Researchs for Behavior Control of Insect Pests based on Host-selection of Insects.

基于昆虫寄主选择的害虫行为控制研究。

基本信息

批准号：
61560051
负责人：
KATO Natsuki
金额：
$ 1.22万
依托单位：
College of Chukyo Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

The researches were performed on a interrelationships between steric conformation and biological activity, and attractants for female and male fruit fly, Daccus cucubitae Cog., in the fruits of Momordica Charantia L.1. It is well known that appearance of biological activity fepend on a steric conformation with ogher factors of bioactive compounds in relation to the structure of the receptor of organism. On based this knowledge, we performed to investigate two steric conformational isomers of teucrin Pl.This terpene compound had a boat conformation on the B and E rings. However, the boat conformation of the E ring chaged to the chair conformer by treatment with LDA/THF-HMPA. The fact was confirmed with NOE experiment and X-ray analyis. Stability of the conformational ismoer was shown on time-corse CD spectra in CHCL3 at room temperature, and the 60% amounts of the isomer had been changed to teucrin Pl for 14 days. We will continue to investigate the change of the biological activity resulting from the conformational change of the B ring.2. We had been founded that linolenoyl glycerol, which showed excellent attractant rantia L. did not showed the attractant acitivty in the field. We performed further to resolve this problem and to inverstigate new attractant from the fruits. From the higher polarity fraction, methyl p-hydroxybenzoate was isolated as the attractnat fro male fruit fly. Furthermore, the attractnats for female and male of frit fly was isolated from the most polarity fraction. This compound was deduced ad 1-3-(<beta>-galactopyranosyl)-2,3-di-o-linolenoyl-x-glycerol on the^<13>C and 2D NMR spectra. From the same fraction, thr new attractant for the female and male fruit fly was isolated and was shown that 6-linolenoyl-<beta>-D-glucopyranosyl(1,3')-<beta>-clerosterol on the 500 MHz and 2D NMR spectra. Now we performe to synthesys these glycoside componds, and on their completion, we well test in the field with linolenoyl glycerol derivatives.

研究了雌雄果蝇的空间构象与生物活性、引诱剂的相互关系。在苦瓜（Momordica Charantia）的果实中。众所周知，生物活性的外观取决于与生物受体结构有关的生物活性化合物的其他因素的立体构象。在此基础上，我们研究了两种构象同分异构体的构象，这种萜类化合物在B环和E环上具有船形构象。然而，经LDA/THF-HMPA处理后，E环的船形构象变为椅形构象。通过NOE实验和x射线分析证实了这一事实。构象异构体在CHCL3中的室温时程CD谱显示了其稳定性，60%的异构体在14天内变成了teucrin Pl。我们将继续研究B环构象变化对生物活性的影响。我们已经发现亚麻油酰甘油在田间没有表现出引诱作用，而亚麻油酰甘油在田间表现出很好的引诱作用。为了进一步解决这一问题，并从果实中研究新的引诱剂。从较高极性部分分离出对羟基苯甲酸甲酯作为雄性果蝇的引诱物。此外，从大多数极性部分分离出了雌蝇和雄蝇的引诱物。该化合物在<13>C和二维核磁共振波谱上通过1-3-（< β >-半乳糖酰基）-2,3-二-o-亚麻烯酰-x-甘油得到。在500 MHz和2D核磁共振谱上分别分离出了6-亚麻烯酰-< β >- d -葡萄糖吡喃基（1,3′）-< β >-胆固醇。现在我们开始合成这些苷类化合物，完成后，我们用亚麻油酰甘油衍生物进行了现场测试。