Researchs for Behavior Control of Insect Pests based on Host-selection of Insects.

研究了苦瓜果实中的立体构象与生物活性的关系以及引诱剂对雌雄果蝇的引诱作用。众所周知，生物活性的出现依赖于与生物体受体结构有关的生物活性化合物的欧格尔因子的空间构象。在此基础上，我们研究了铁皮苷P的两种立体构象异构体，这种萜类化合物在B环和E环上具有船形构象。但经LDA/THF-HMPA处理后，E环的船型构象改变为座椅构象。NOE实验和X射线分析证实了这一事实。构象异构体在CHCl_3中的时间相关圆二色谱表明其在室温下是稳定的，其中60%的异构体在14天内转变为茶树苷Pl。我们将继续研究B环构象变化对生物活性的影响。我们发现，具有优良引诱剂的亚麻油甘油在田间没有表现出引诱活性。我们做了进一步的工作来解决这个问题，并从水果中寻找新的引诱剂。从较高的极性组分中分离出对羟基苯甲酸甲酯对雄性果蝇具有引诱作用。此外，还从最大极性组分中分离出对雌、雄蝇的引诱作用。该化合物由~1t；13&gt；C和2D核磁共振波谱推断为ad 1-3-(&lt；beta&gt；-galactopyranosyl)-2，3-di-o-linolenoyl-x-glycerol。从同一组分中分离得到一种新的引诱剂，其对雌雄果蝇均有引诱作用，并在500 MHz和2D核磁共振波谱上证明了6-linolenoyl-&lt；beta&gt；-D-glucopyranosyl(1，3‘)-&lt；beta&gt；-clerosterol。现在我们合成了这些糖苷类化合物，在它们完成后，我们在现场用亚麻油酰甘油衍生物进行了很好的测试。

The researches were performed on a interrelationships between steric conformation and biological activity, and attractants for female and male fruit fly, Daccus cucubitae Cog., in the fruits of Momordica Charantia L.1. It is well known that appearance of biological activity fepend on a steric conformation with ogher factors of bioactive compounds in relation to the structure of the receptor of organism. On based this knowledge, we performed to investigate two steric conformational isomers of teucrin Pl.This terpene compound had a boat conformation on the B and E rings. However, the boat conformation of the E ring chaged to the chair conformer by treatment with LDA/THF-HMPA. The fact was confirmed with NOE experiment and X-ray analyis. Stability of the conformational ismoer was shown on time-corse CD spectra in CHCL3 at room temperature, and the 60% amounts of the isomer had been changed to teucrin Pl for 14 days. We will continue to investigate the change of the biological activity resulting from the conformational change of the B ring.2. We had been founded that linolenoyl glycerol, which showed excellent attractant rantia L. did not showed the attractant acitivty in the field. We performed further to resolve this problem and to inverstigate new attractant from the fruits. From the higher polarity fraction, methyl p-hydroxybenzoate was isolated as the attractnat fro male fruit fly. Furthermore, the attractnats for female and male of frit fly was isolated from the most polarity fraction. This compound was deduced ad 1-3-(<beta>-galactopyranosyl)-2,3-di-o-linolenoyl-x-glycerol on the^<13>C and 2D NMR spectra. From the same fraction, thr new attractant for the female and male fruit fly was isolated and was shown that 6-linolenoyl-<beta>-D-glucopyranosyl(1,3')-<beta>-clerosterol on the 500 MHz and 2D NMR spectra. Now we performe to synthesys these glycoside componds, and on their completion, we well test in the field with linolenoyl glycerol derivatives.