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Taxonomical and Ecological Studies on the Causative Dinoflagellates of Diarretic Shellfish Poisoning.

引起腹泻性贝类中毒的甲藻的分类学和生态学研究。

基本信息

批准号：
61560204
负责人：
ISHIMARU Takashi
金额：
$ 1.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560204/
关键词：
Diarretic Shellfish Poisoning (DSP)Dinoflagellate Taxonomy Culture ディノフィシス

项目摘要

Diarrehtic shellfish poisoning (DSP) is intoxification of shellfish which feed on dinoflagellates, Dinophysis spp.. DSP causes extensive impacts on fisheries industries in Japan as well as in European countries. As nobody have ever suceeded in culturing Dinophysis spp., physiological and ecological character of Dinophysis and the mechanism of the toxin production by the species are still unclear. Purposes of the present study are to make clear the nutitional style of Dinophysis and to establish culture method for them.At a first step, osmotrophic growth was tested. Cells of D. fortii were inoculated to enriched seawater media containing various organic compaunds. No growth were observed in axenic condition but some cells were survived in media contaminated with bacteria. Growth was also observed in seawater filterd by plankton net containing microflagellates. Thus, osmotrophy of D. fortii was denied but Phagotrophy was sugested. Then, Phagotrophic growth was tested by culturing D.fortii, D. acuminata and D. caudata with various species of micro algae as foods. As these Dinophysis species have orange fluorescence of phycobilin, cyanobacter or cryptomonad were thought to be contained in the cells of them. A cryptomonad, Plagioselmis sp. was observed to taken into the cells of 3 Dinophysis spp.. Growth of D. fortii and D. caudata were observed in culturing with Plagioselmis sp., but did not increase in large cell number. In order to establish cultures of Dinophysis spp., It is needed to study other factors which affect the growth of the species.

腹泻性贝类中毒（DSP）是一种以甲藻、甲藻为食的贝类中毒。DSP对日本和欧洲国家的渔业造成了广泛的影响。由于目前还没有人成功地培育出这种鱼，因此对其生理生态特性和产毒机制尚不清楚。本研究旨在明确恐龙的营养类型，建立恐龙的培养方法。在第一步，渗透生长进行了测试。将福氏弓形虫细胞接种于含有多种有机化合物的富营养化海水培养基中。无菌条件下未见细胞生长，但有部分细胞在细菌污染的培养基中存活。在含有微鞭毛虫的浮游生物网过滤的海水中也观察到生长。因此，富特氏菌的渗透作用被否认，而吞噬作用被提出。然后，以不同种类的微藻为食，分别培养fortii、acuminata和尾状D.，考察其吞噬生长情况。由于这些恐龙具有藻胆素的橙色荧光，因此认为它们的细胞中含有蓝藻杆菌或隐单胞菌。观察到一种隐孢子虫Plagioselmis sp.被带进3种恐龙的细胞中。与斜交菌共同培养时，可观察到fortii和尾尾d的生长，但细胞数量没有显著增加。为了建立恐龙藻的培养，需要对影响该物种生长的其他因素进行研究。