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Studies on the in vitro maturation of goat testicular spermatozoa

山羊睾丸精子体外成熟的研究

基本信息

批准号：
61560307
负责人：
KATO Seishiro
金额：
$ 1.22万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

1.Factors affecting the motility of testicular spermatozoaSome 30-40% of testicular spermatozoa exhibited only feeble flagellar oscillation either after collection or after being washed and resuspended in a Krebs Ringer bicarbonate solution. After the addition of caffeine(25mM) to samples the percentages of spermatozoa showing vigorous oscillating or circular movements increased to approximately 90%, although forward progress was still extremely limited. However, when an aliquot of seminal plasma, cauda epididymal plasma, extracts from the accessory genital organs (ampulla of vas deferens, seminal vesicle, prostate) and nonreproductive organs, or egg yolk was added along with caffeine, progressive motility was induced in a proportion (10-60%) of testicular spermatozoa. These spermatozoa showed the rotatory movement so typical of fully matured cells but forward progress was much slower. Maximum progressive motility-inducing activity was observed with seminal plasma. The activities of ex … More tracts from the seminal vesicle and ampulla were also higher than those for cauda epididymal fluid, extracts from nonreproductive organs, and egg yolk. These results suggest that testicular spermatozoa already have a basic structure for progressive motility.2.Induction of the acrosome reaction in testicular spermatozoaTesticular spermatozoa were preincubated for 5 hr in the isolated hamster uteri and incubated with zona-free hamster eggs. Of 22 eggs, 36 (15.5%) were penetrated by spermatozoa. The proportion of acrosome-reacted spermatozoa in samples preincubated under similar conditions was 18.2% as assessed by a simplified triple stain technique. These results suggest that testicular spermatozoa can undergo the acrosome teaction and aquire the ability to penetrate zona-free eggs.3.Changes in motility, morphology and fertilizing ability of goat spermatozoa during transit through the epididymis, storage of testicular spermatozoa, concentrations of steroid hormones in rete testicular fluid, and histological changes in the testis and caput epididymis after cannulation of the rete testis were also examined. Less

1.影响睾丸精子运动的因素约30-40%的睾丸精子在收集后或洗涤后重悬于Krebs Ringer碳酸氢盐溶液中仅显示微弱的鞭毛振荡。在向样品中加入咖啡因（25 mM）后，表现出剧烈振荡或圆形运动的精子百分比增加到约90%，尽管向前的进展仍然非常有限。然而，当将一等份精浆、附睾尾浆、辅助生殖器官（输精管壶腹、精囊、前列腺）和非生殖器官的提取物或蛋黄与咖啡因一起沿着加入时，在一定比例（10-60%）的睾丸精子中诱导了前向运动。这些精子表现出完全成熟细胞的典型旋转运动，但向前运动的速度要慢得多。在精浆中观察到最大的前向运动诱导活性。前的活动 ...更多信息贮精囊和壶腹液的含量也高于附睾尾液、非生殖器官提取物和蛋黄。睾丸精子顶体反应的诱导将睾丸精子在离体仓鼠子宫中预孵育5小时，然后与去透明带的仓鼠卵共同孵育。在22个卵中，36个（15.5%）被精子穿透。在类似条件下预孵育的样品中，顶体反应精子的比例为18.2%，通过简化的三重染色技术进行评估。这些结果表明，山羊睾丸精子可以通过顶体反应，获得穿透无透明带卵子的能力。3.研究了山羊精子在附睾中运动、形态和可复性的变化，睾丸精子的贮存，睾丸网液中类固醇激素的含量，以及睾丸和附睾头在睾丸网插管后的组织学变化。少