Studies on the in vitro maturation of goat testicular spermatozoa

1.影响睾丸精子活力的因素约30-40%的睾丸精子在收集后或在Krebs Ringer碳酸氢盐溶液中洗涤和再悬浮后，仅表现出微弱的鞭毛振荡。在样品中加入咖啡因(25 MM)后，显示剧烈振荡或环形运动的精子百分比增加到约90%，尽管前进的进展仍然极其有限。然而，当加入等量的精浆、附睾尾血浆、辅助生殖器官(输精管壶腹、精囊、前列腺)和非生殖器官的提取物，或蛋黄与咖啡因一起加入时，可诱导一定比例(10%-60%)的睾丸精子向前运动。这些精子表现出完全成熟细胞的典型旋转运动，但前进的速度要慢得多。在精浆中观察到最大的递增运动诱导活性。前…的活动精囊和壶腹的数量也高于附睾尾液、非生殖器官提取液和蛋黄。这些结果表明，睾丸精子已经具有前进运动的基本结构。2.睾丸精子顶体反应的诱导。将睾丸精子在分离的金黄地鼠子宫中预培养5小时，并与去透明带地鼠卵孵育。在22枚卵子中，36枚(15.5%)被精子穿透。用简化的三染色法测定，在相似条件下预培养的样品中，顶体反应的精子比例为18.2%。这些结果表明，山羊精子可以通过顶体作用获得穿透去透明带卵子的能力。3.还观察了山羊精子在附精过程中的活力、形态和受精能力的变化，睾丸精子的保存，睾丸网液中类固醇激素的浓度，以及睾丸和附睾头的组织学变化。较少

1.Factors affecting the motility of testicular spermatozoaSome 30-40% of testicular spermatozoa exhibited only feeble flagellar oscillation either after collection or after being washed and resuspended in a Krebs Ringer bicarbonate solution. After the addition of caffeine(25mM) to samples the percentages of spermatozoa showing vigorous oscillating or circular movements increased to approximately 90%, although forward progress was still extremely limited. However, when an aliquot of seminal plasma, cauda epididymal plasma, extracts from the accessory genital organs (ampulla of vas deferens, seminal vesicle, prostate) and nonreproductive organs, or egg yolk was added along with caffeine, progressive motility was induced in a proportion (10-60%) of testicular spermatozoa. These spermatozoa showed the rotatory movement so typical of fully matured cells but forward progress was much slower. Maximum progressive motility-inducing activity was observed with seminal plasma. The activities of ex … More tracts from the seminal vesicle and ampulla were also higher than those for cauda epididymal fluid, extracts from nonreproductive organs, and egg yolk. These results suggest that testicular spermatozoa already have a basic structure for progressive motility.2.Induction of the acrosome reaction in testicular spermatozoaTesticular spermatozoa were preincubated for 5 hr in the isolated hamster uteri and incubated with zona-free hamster eggs. Of 22 eggs, 36 (15.5%) were penetrated by spermatozoa. The proportion of acrosome-reacted spermatozoa in samples preincubated under similar conditions was 18.2% as assessed by a simplified triple stain technique. These results suggest that testicular spermatozoa can undergo the acrosome teaction and aquire the ability to penetrate zona-free eggs.3.Changes in motility, morphology and fertilizing ability of goat spermatozoa during transit through the epididymis, storage of testicular spermatozoa, concentrations of steroid hormones in rete testicular fluid, and histological changes in the testis and caput epididymis after cannulation of the rete testis were also examined. Less