Melanin-concentrating hormone-like immunoreactive substance in the rat hypothalamus.

大鼠下丘脑中的黑色素浓缩激素样免疫反应物质。

• 批准号: 61570032
• 负责人: NAITO Nobuko
• 金额: $ 1.28万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570032/
• 关键词: Melanin-concentrating hormone (MCH); <alpha>-MSH; Rat; Hypothalamus; Neuropeptide coexistence; Immunohistochemistry; Radioimmunoassay; バイオアッセイ; 神経ペプチド; 免疫組職化学

Melanin-concentrating hormone (MCH) is a neuropeptide synthesized in the teleost hypothalamus and stimulates melanin aggregation within teleost melanophores. In the rat brain, however, MCH-bioactivity and immunoreactivity have been reported independently.In the present project, we characterized MCH-like substance in the rat brain by both an in vitro fish-scale melanophore-bioassay and a radioimmunoassay (RIA) with an antiserum against salmon MCH. Futhermore, subcellular localization of the MCH in the rat brain was examined by immunocytochemistry using electron microscopy. 1. Structure-activity relationships of the salmon MCH molecule were confirmed. MCH-bioactivity requires the disulfide bonding between cystein and amino acid residues (Tyr^<11> and Arg^<12>). Salmon MCH antiserum used in our studies is directed towards the carboxy-terminus and requires the cyclic configuration for recognition. 2. MCH-immunoreactivity and -bioactivity were present together in the same effluent fractions of the rat hypothalamic extract by reverse-phase high-performance liquid chromatography (HPLC). 3. MCH-immunoreactive cell bodies in the rat brain were confined to the hypothalamus. At electron microscopic level, MCH-immunoreactivity was located specifically in secretory granules in MCH-positive neurons and their processes. 4. Coexistence of immunoreactivity for MCH and -MSH was presented in the hypothalamus of the rat.Our results strongly upport the notion that rat MCH-like substance may be homologous but not identical to salmon MCH, and may serve some neurotransmitter and/or neuromodulator role in the brain of the rat.

黑色素浓缩激素（melanin -concentrating hormone， MCH）是一种在硬骨鱼下丘脑合成的神经肽，并刺激硬骨鱼黑色素细胞内的黑色素聚集。然而，在大鼠脑中，mch的生物活性和免疫反应性已被独立报道。在本项目中，我们通过鱼鳞黑色素细胞体外生物测定和鲑鱼MCH抗血清放射免疫测定（RIA）对大鼠脑中的MCH样物质进行了表征。利用电镜免疫细胞化学方法检测MCH在大鼠脑中的亚细胞定位。1. 证实了三文鱼MCH分子的构效关系。mch生物活性需要半胱氨酸和氨基酸残基之间的二硫键（Tyr^<11>和Arg^<12>）。在我们的研究中使用的鲑鱼MCH抗血清是针对羧基端，需要环状结构的识别。2. 通过反相高效液相色谱法（HPLC），在大鼠下丘脑提取物的同一流出物中同时存在mch -免疫反应性和-生物活性。3. 大鼠脑中的mch免疫反应细胞体局限于下丘脑。在电镜下，mch阳性神经元及其过程的分泌颗粒特异性地表达了mch免疫反应性。4. 大鼠下丘脑出现MCH和-MSH共存的免疫反应性。我们的研究结果有力地支持了大鼠MCH样物质可能与鲑鱼MCH同源但不完全相同的观点，并且可能在大鼠大脑中发挥某种神经递质和/或神经调节剂的作用。

项目成果

NAITO,Nobuko: Neurosci.Lett.70. 81-85 (1986)

内藤信子：神经科学.快报.70。

NAITO,Nobuko: "Demonstration of the hypothalamo-hypophysial connection in the teleost brain with an antiserum to the melanin-concentrating hormone." In "Pars Distalis of the Pituitary Gland-Structure, Function and Regulation" (ed. Yoshimura, F. and Gorbma

NAITO,Nobuko：“用黑色素浓缩激素抗血清证明硬骨鱼大脑中下丘脑-垂体的联系。”

NAITO,Nobuko eds.Yoshimura,F.and Gorbman,A.: IN"Pars Distalis of Pituitary Gland-Structure,Function and Regulation". 255-257 (1986)

NAITO,Nobuko eds.Yoshimura,F.和 Gorbman,A.：IN“垂体远端部的结构、功能和调节”。

NAITO, Nobuko: "Coexistence of immunoreactivity for melanin-concentrating hormone and -melanocyte-stimulating hormone in the hypothalamus of the rat." Neurosci. Lett.70. 81-85 (1986)

NAITO，Nobuko：“大鼠下丘脑中黑色素浓缩激素和黑色素细胞刺激激素的免疫反应性共存。”

KAWAZOE,Ichiro: Int.J.Peptide Protein Res.29. 714-721 (1987)

KAWAZOE，Ichiro：Int.J.肽蛋白研究29。