Studies on the effect of gastro-intestinal, pancreatic and cardiac hormones on the efferent nerve activity of the autonomic nerve.

在麻醉下本实验观察了静脉注射五肽胃泌素（500-800 mg/kg乌拉坦和40- 70 mg/kg氯醛糖）、CCK-8、生长抑素和心钠素（ANP）对大鼠自主神经传出活动的影响，发现注射五肽胃泌素后，迷走神经胃分支活动增强，应用生长抑素或ANP后，迷走神经胃支、肝支和胰分支的活动均降低。应用ANP还可抑制肾上腺和肾交感神经传出活动。这些物质的最小有效剂量为100 pg.在去大脑标本中，给药后未观察到传出活动的变化.结果表明，释放到静脉循环中的神经肽如五肽胃泌素、CCK-8、生长抑素和ANP通过血脑屏障不足的下丘脑神经元网络调节自主神经流出。

Under anaesthesia (urethane 500-800 mg/kg and chloralose 40-70mg/kg), effect of i.v. administration of pentagastrin, CCK-8, somatostatin and ANP on efferent activity of autonomic nerve was observed in rats.An increase in activity was observed in gastric branch of vagus nerve following injection of pentagastrin, and that in pancreatic branch of vagus nerve after injection of CCK-8.A decrease in activity was observed in gastric, hepatic and pancreatic branch of vagus nerve following application of somatostation or ANP. ANP application also caused a suppression on activity of adrenal and renal sympathetic efferents. The smallest effective dose of these substances was 100pg.In decerebrated preparations no change in efferent activity was observed after administrations of these substances.The results indicate that neuropeptides such as pentagastrin, CCK-8, somatostatin and ANP released into venous circulation modulate autonomic outflows through hypothalamic neuronal networks surrounding with insufficient blood-brain barrier.