Studies on Calcium-activated, Unsaturated Fatty acid-dependent Guanylate Cyclase in Membranes.

膜中钙激活、不饱和脂肪酸依赖性鸟苷酸环化酶的研究。

• 批准号: 61570129
• 负责人: ASAKAWA Takeo
• 金额: $ 1.09万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570129/
• 关键词: Guanylate Cyclase; Synaptic Plasma Membrane; Ca^<2+>; Unsaturated Fatty Acid; Unsaturated Fatty Acid; 調節因子; 【Ca^(2+)】

Cyclic GMP in an intact cell system was reported to increase by various biologically active compounds as neurotransmitters and hormones. Guanylate Cyclase in cell-free system can not be stimurated by agents increasing cyclic GMP contents. A mechanism of receptor-operated enhancement of cyclic GMP formation has been unclear. Recently we described the Ca^<2+>-activated, unsaturated fatty acid-dependent Guanylate Cyclase (CF-Guanylate Cyclase) in synaptic plasma membranes. This enzyme system plays possibly an important functional role in transmembrane-signal-transduction.The stimulatory substances of the cyclase, Ca^<2+> and unsaturated fatty acid which are librated intramembranously on receptor-stimulation are proposed to be a mediator probably participating in a receptor-guanylate cyclase coupling. In this study, we examined purification procedures and some properties of CF-Guanylate Cyclase.The mechanism of the activation of this cyclase by Ca^<2+> and fatty acid was partly clarified. In chromatographies of this enzyme, a factor, we named it CF-Guanylate Cyclase modulator (GCM) was separated from the enzyme preparation, which significantly modulated the activation by fatty acids. The factor was purified by several steps of chromatographies and estimated to be approx. 37K. The properties of the factor is now under investigating.

据报道，完整细胞系统中的环GMP可通过各种生物活性化合物（如神经递质和激素）增加。无细胞体系中鸟苷酸环化酶不能被增加环鸟苷酸含量的物质所刺激。受体操作的环鸟苷酸形成的增强机制尚不清楚。最近，我们描述了突触质膜中Ca^2+激活的不饱和脂肪酸依赖性鸟苷酸环化酶（CF-鸟苷酸环化酶）。该酶系统可能在跨膜信号转导中起重要作用，环化酶的刺激物质Ca^（2+）和不饱和脂肪酸可能参与受体-鸟苷酸环化酶偶联反应。本文研究了CF-鸟苷酸环化酶的纯化方法和部分性质，初步阐明了Ca^<2+>和脂肪酸对该环化酶的激活机制。在该酶的层析中，从酶制剂中分离出一种因子，我们将其命名为CF-鸟苷酸环化酶调节剂（GCM），它显著地调节脂肪酸的活化。该因子通过几个步骤的色谱法纯化，估计约为。三万七该因子的性质目前正在研究中。

项目成果

麻川武雄: 生化学. 58. 1072 (1986)

浅川武夫：生物化学 58. 1072 (1986)

K.Enomoto.: Eur.J.Biochem.(1988)

K.Enomoto.：Eur.J.Biochem.(1988)

M.Takano: Jpn.J.Pharmacol.44sup.185 (1987)

M.Takano：Jpn.J.Pharmacol.44sup.185 (1987)

榎本恵一: 神経化学. 25. 154-156 (1986)

榎本敬一：神经化学 25. 154-156 (1986)

M.Higuchi.: Jpn;J.Pharmacol.44. 145-153 (1987)

M.Higuchi.：Jpn；J.Pharmacol.44。