Role of hepatic cell biomembrane-cytoskelton system in development of triglyceride fatty livers.

在注射乙硫氨酸后3、5、9和12小时，对禁食雌性大鼠的肝脏进行了超微结构观察。特别是为了研究甘油三酯脂肪肝的早期阶段，我们用我们的酶消化法(Acta组织化学.细胞化学15：185,1982和J组织化学.细胞化学.31：150,1983)对早期收获的肝脏进行了检查。在内质网内的许多微小脂肪颗粒中可以明显地发现甘油三酯定位的反应产物，尽管当时肝组织上甘油三酯的生化测定并没有显著增加。为了观察乙硫氨酸损伤肝脏上细胞质内脂质包裹体的发展过程，随后采集的肝脏用我们的整块铅染色(Proc.XIth Int.Cong.电子显微镜：2189,1986)。整块染色块的超薄切片提供了更高对比度的电子显微镜图像，显示了CLI的精确定位，以前没有报道过…伪影用这种特殊的电子显微镜不能显示上述生物膜结合的脂质颗粒(BLP)向CLI的转化。用磷脂酶消化法观察了上述实验大鼠的肝组织块和肝微粒组分颗粒，推测新出现的含有反应产物的沉淀物是某些细胞质内膜磷脂消化的结果。成年雌性大鼠隔夜饥饿并在禁食第11小时注射乙硫氨酸(1g/kg)。其中一半动物在注射乙硫氨酸后3小时和6小时两次注射秋水仙碱(2.5 mg/kg)。禁食20小时后处死动物，用电子显微镜观察肝门周围的肝细胞。在乙硫氨酸和秋水仙碱双重注射的大鼠中，CLI明显大于单独注射乙硫氨酸的大鼠。前者的肝细胞CLI低于后者，但两组大鼠肝脏总脂的升高相似。这些结果表明秋水仙碱抑制乙硫氨酸损伤的雌性大鼠肝脏CLI的发展。这种作用可能与微管解聚或直接代谢作用有关，有待进一步研究。较少

The livers of fasting female rats were observed ultrastructurally 3,5,9 and 12 hr after an ethionine injection.Especially,to investigate an early stage of triglyceride fatty liver,the earlier harvested liver was examined with our enzymic digestive methods(Acta histochem.cytochem.15:185, 1982 and J.Histochem.Cytochem.31:150,1983).The reaction products indicating the location of triglycerides could be found distinctly in many of minute lipid particles within ER,although no significant increase in the biochemic determination of triglycerides on the hepatic tissues could be noted at the time.To check the developing process of intracytoplasmic lipid inclusions(CLI)on the ethionine-injured livers,the later harvested livers were treated with our en bloc staining by lead (Proc.XIth Int.Cong.Electron Microscopy:2189,1986).The ultrathin sections of the en bloc staining blocks provided a higher contrasted electron micrograph showing precise localization of CLI and no artifacts previously reported … More by us(J.Kansai Med.Univ.37 Suppl:54,1985).The transformation from the above-mentioned biomembrane-bound lipid particles(BLP)to CLI could not be demonstrated with this special electron microscopy.Both hepatic tissue blocks and pellets of liver microsome fractions from the above-mentioned experimental rats were examined with phospholipase digestive methods,and newly appearing deposits containing the reaction products were speculated to result from the digestion of some intracytoplasmic membrane-phospholipids.Adult female rats were starved overnight and injected with ethionine(1g/Kg)at the 11th hr of fasting,and then a half of them was also injected with colchicine(2.5mg/Kg)twice at 3 and 6 hr after an ethionine injection.All animals were sacrificed after having fasted a total of 20 hr,and the hepatocytes in periportal area were observed with electron microscopy.In addition,the liver lipids of these animals were extracted and analyzed biochemically.The accumulation of larger BLP(diameter; 0.2-0.4 <micrn>m mainly)was greater in the rats injected doubly with ethionine and colchicine than in the rats injected only with ethionine.Such hepatocytes of the former group had fewer CLI than the latter group,although the livers of both groups exhibited similar rises in liver total lipids.These findings indicate that colchicine inhibits the development of CLI in the ethionine-injured livers of fasting female rats.The effect may be due to the microtubule depolymerization or the derect metabolic action.Further investigations should be done. Less