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Role of hepatic cell biomembrane-cytoskelton system in development of triglyceride fatty livers.

肝细胞生物膜-细胞骨架系统在甘油三酯脂肪肝发展中的作用。

基本信息

批准号：
61570183
负责人：
MORII Sotokichi
金额：
$ 1.22万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

The livers of fasting female rats were observed ultrastructurally 3,5,9 and 12 hr after an ethionine injection.Especially,to investigate an early stage of triglyceride fatty liver,the earlier harvested liver was examined with our enzymic digestive methods(Acta histochem.cytochem.15:185, 1982 and J.Histochem.Cytochem.31:150,1983).The reaction products indicating the location of triglycerides could be found distinctly in many of minute lipid particles within ER,although no significant increase in the biochemic determination of triglycerides on the hepatic tissues could be noted at the time.To check the developing process of intracytoplasmic lipid inclusions(CLI)on the ethionine-injured livers,the later harvested livers were treated with our en bloc staining by lead (Proc.XIth Int.Cong.Electron Microscopy:2189,1986).The ultrathin sections of the en bloc staining blocks provided a higher contrasted electron micrograph showing precise localization of CLI and no artifacts previously reported … More by us(J.Kansai Med.Univ.37 Suppl:54,1985).The transformation from the above-mentioned biomembrane-bound lipid particles(BLP)to CLI could not be demonstrated with this special electron microscopy.Both hepatic tissue blocks and pellets of liver microsome fractions from the above-mentioned experimental rats were examined with phospholipase digestive methods,and newly appearing deposits containing the reaction products were speculated to result from the digestion of some intracytoplasmic membrane-phospholipids.Adult female rats were starved overnight and injected with ethionine(1g/Kg)at the 11th hr of fasting,and then a half of them was also injected with colchicine(2.5mg/Kg)twice at 3 and 6 hr after an ethionine injection.All animals were sacrificed after having fasted a total of 20 hr,and the hepatocytes in periportal area were observed with electron microscopy.In addition,the liver lipids of these animals were extracted and analyzed biochemically.The accumulation of larger BLP(diameter; 0.2-0.4 <micrn>m mainly)was greater in the rats injected doubly with ethionine and colchicine than in the rats injected only with ethionine.Such hepatocytes of the former group had fewer CLI than the latter group,although the livers of both groups exhibited similar rises in liver total lipids.These findings indicate that colchicine inhibits the development of CLI in the ethionine-injured livers of fasting female rats.The effect may be due to the microtubule depolymerization or the derect metabolic action.Further investigations should be done. Less

本文观察了禁食雌性大鼠注射乙撑双胍后3、5、9和12小时的肝脏超微结构，特别是为了探讨甘油三酯脂肪肝的早期阶段，用我们的酶消化法检查了早期收获的肝脏（Acta histochem.cytochem.15：185，1982和J.Histochem.Cytochem.31：150，1983）。在ER内的许多微小脂质颗粒中可以清楚地发现指示甘油三酯位置的反应产物，尽管当时在肝组织上的甘油三酯的生化测定中没有显著增加。为了检查乙硫氨酸损伤的肝脏上的胞浆内脂质包涵体（CLI）的发展过程，用我们的铅整体染色（Proc. XIthInt.Cong. ElectronMicroscopy：2189，1986）处理后取的肝脏，整体染色块的X射线切片提供了对比度更高的电子显微照片，显示CLI的精确定位，没有以前报道的伪影 ...更多信息美国（J.Kansai Med.Univ.37增刊：用磷脂酶消化法检查上述实验大鼠的肝组织块和肝微粒体部分的颗粒，推测新出现的含有反应产物的沉积物是由于细胞质膜内磷脂的消化所致。然后其中一半也注射了秋水仙碱（2.5mg/Kg）两次，在总共禁食20小时后处死所有动物，电镜观察门静脉周围肝细胞，并提取肝脏脂质进行生化分析，发现肝内有较大的BLP（直径0.2-0.4 <micrn>μ m为主）聚集，肝细胞内有大量的BLP沉积，肝结果表明，乙醇胺和秋水仙碱双重注射组大鼠肝细胞的CLI明显高于单纯注射组，前者肝细胞的CLI明显低于后者，尽管两组的肝脏显示出相似的肝脏总脂质升高。这些发现表明秋水仙素抑制了乙醇中毒中CLI的发展，结果表明，本品对禁食雌性大鼠肝脏有明显的损伤作用，其作用可能是由于微管解聚或直接的代谢作用，有待进一步研究。少