Studies on blackflies in relation to their laboratory colonization.

对几种日本黑蝇在实验室定植的可能性进行了研究。结果，在这些物种中，只有一种被证明是交配的，以人、兔和狗的血液为食，并在实验室产卵。在此基础上，对成虫的交配和授精历时、血粉大小对卵成熟的影响、交配或温度对产卵的影响以及多个生殖营养循环等进行了较为详细的研究，并观察了胚胎发育和产卵孵化，用搅拌棒系统成功地将孵化出的幼虫发育到成虫阶段。此外，在实验室中还发现了野外采集的其他种类的雌性黑蝇(原黑蝇、青海黑蝇、阿拉卡瓦黑蝇、日本黑蝇、红小黑蝇)在实验室产卵，尽管这些物种从未被观察到在圈养中交配。在不同水温条件下，所有这些物种的发育都是使用相同的搅拌棒系统完成的，只有基氏拟青蟹的卵发育到成虫。金黄色葡萄球菌的第一次卵母细胞成熟无需补血，但需要糖液才能完成。

The possibility of laboratory colonization was examined with several Japanese blackfly species. As a result, only one of these species, Simulium takahasii, was proved to mate, feed on blood form man, rabbit and dog, and lay eggs in the laboratory. The biology of adult of this species was then studied in more detail, e. g., the duration needed for mating and insemination, the effect of blood-meal size on egg maturation, the effect of mating or temperature on oviposition, and multiple gonotrophic cycles, etc. The embryonic development and hatching of laid eggs were observed, with the successful subsequent development of hatched larvae to adult stage, suing a stir-bar system. In addition, field-collected gravid females of other blackfly species (Prosimulium kiotoense, S. aokii, S. arakawae, S. japonicum, S. nikkoense, S. rufibasis) were found to oviposit in the laboratory, although these species were never observed to mate in captivity. The development of all these species but P. kiotoense form the eggs to adults was accomplished using the same stir-bar system under various water temperatures. The first oocyte maturation of S. aureohirtum underwent without blood feeding, but its completion required sugar solution.