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Analysis on the expression of protection to Listeria monocytogenes by MCF derived from T cells with a low thymus dependency

低胸腺依赖性T细胞MCF对单增李斯特氏菌的保护作用分析

基本信息

批准号：
61570213
负责人：
MITSUYAMA Masao
金额：
$ 1.34万
依托单位：
Niigata University (1987)Kyushu University (1986)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

So-called cell-mediated immunity has been regarded as a thymus-dependent immunity, however, there are various immune responses which can be easily mounted in neonatally thymectomized (NTx) mice but not in nude mice. Protective immunity to Listeria monocytogenes is an example of such an immunity with a low thymus dependency.In the present study, the characterization of protective T cells against L.monocytogenes, which are of low thymus dependency, was done. Among various lymphokins produced by Listeria-immune T cells, MCF was of the special interest in this study, since the biological significance of this lymphokine in the expression of protection has not yet been elucidated. T cells obtained from NTx mice immunized with viable L.monocytogenes was shown to be Lytl^+,L3T4^+ and Lyt2^- cells. The effector T cells could release several lymphokines upon stimulation with specific antigen; however, they showed an impaired release of IL-2 and antigen-specific proliferation. It was suggested th … More at the characetristics of T cells with a low thymus dependency may be a low requirement for clonal expansion. Using the culture supernatant of Listeriaimmune T cells plus antigen, a partial purification of MCF was carried out by a gel chromatography. Fractions showing MCF activity but no contaminating MAF activity could be obtained. When this MCF fraction was injected into mice, a marked accumulation of macrphages was observed 10 h after injection. MCF-induced accumulation of macrophages was effective against challenge with relatively small dose of virulent strain and with even a high dose of low virulent strain of L.monocytogenes. The most significant level of protection was observed in the footpad given MCF, probably because of the localization of MCF at this site. When IFN-<gamma> was used as MAF in vivo, the highest level of protection was obtained by the use of an additional administration of MCF. These results suggested that MCF, which is produced by T cells with a low thymus dependency, plays an important role by itself in the expression of protective immunity and also is capable of showing the synergistic effect with MAF/IFN-<gamma>. Less

所谓的细胞免疫一直被认为是胸腺依赖性免疫，然而，存在多种免疫应答，这些免疫应答可以容易地在切除胸腺（NTx）的小鼠中产生，而在裸鼠中不产生。对单核细胞增生李斯特菌的保护性免疫是低胸腺依赖性免疫的一个例子，本研究对低胸腺依赖性的单核细胞增生李斯特菌保护性T细胞进行了表征。在李斯特菌免疫T细胞产生的各种淋巴因子中，MCF在本研究中特别感兴趣，因为这种淋巴因子在保护表达中的生物学意义尚未阐明。从用活的单核细胞增多性李斯特菌免疫的NTx小鼠获得的T细胞显示为Lytl^+、L3 T4 ^+和Lyt 2 ^-细胞。效应T细胞可以释放几种淋巴因子的刺激与特定的抗原，但是，他们表现出受损的释放IL-2和抗原特异性增殖。有人建议， ...更多信息具有低胸腺依赖性的T细胞的特征可能是克隆扩增的低要求。用李斯特氏菌免疫T细胞培养上清加抗原，经凝胶层析法部分纯化MCF。可以获得显示MCF活性但没有污染MAF活性的级分。当将该MCF组分注射到小鼠体内时，注射后10小时观察到巨噬细胞的显着积聚。MCF-诱导的巨噬细胞的积累是有效的，对挑战相对小剂量的强毒株，甚至与高剂量的低毒力菌株的单核细胞增生李斯特菌。在给予MCF的足垫中观察到最显著的保护水平，可能是因为MCF在该部位的定位。当IFN-<gamma>被用作MAF在体内，最高水平的保护是通过使用一个额外的管理MCF。这些结果表明，MCF，这是一个低胸腺依赖性的T细胞产生的，它本身在保护性免疫的表达中起着重要的作用，也能够显示与MAF/IFN-γ的协同效应<gamma>。少