Correlation between fecal bile acid and diet in carcinogenesis of the colon.

粪便胆汁酸与饮食在结肠癌发生中的相关性。

• 批准号: 61570353
• 负责人: MAKINO Takashi
• 金额: $ 1.28万
• 依托单位: School of Medicine, Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570353/
• 关键词: Fecal Bile Acid; Colon Cancer; Gas Chromatography; 高速液体クロマトグラフィー; 胆汁酸; 糞便

Animal model experiments indicate that deoxycholic acid and lithocholic acid act as colon tumor promoters to an extablished large bowel animal carcinogen. The correlation between becal bile acid excretion and diet was studied in patients with colon cancer. Total bile acid excretion in patients with colon cancer were higher than taht in patients with peptic ulcer and colon polyp. In patients with colon cancer, cholic acid and chenodeoxycholic acid excretion after admission were decreased on the hospital diet. When patients were on low fat diet, total bile acid excretion was decreased. It was found that the hospital diet altered the composition and amount of fecal bile acids in patients with colon cancer. These results were analized by gas chromatography. In this time, fecal bile acids were tried to analize by high pressure liquid chromatography ( HPLC) . Samples were injected for HPLC using Shimazu instrument (RF-530) with Zorbax C8 column. Individual bile acid was analyzed for cholic acid, chenodeoxycholic acid, deoxycholic acid and lithocholic acid. But bile acide could not be analyzed by HPLC. It should seem that analysis of fecal bile acid by gas chromatography is better than that by HPLC in this experiment.

动物模型实验表明，脱氧胆酸和石胆酸作为结肠肿瘤促进剂，以确定大肠动物致癌物。本文研究了结肠癌患者胆汁酸排泄量与饮食的关系。结肠癌患者总胆汁酸排泄量高于消化性溃疡和结肠息肉患者。在结肠癌患者中，住院后的胆酸和鹅去氧胆酸排泄减少。当患者接受低脂饮食时，总胆汁酸排泄减少。研究发现，医院饮食改变了结肠癌患者粪便胆汁酸的组成和数量。用气相色谱法对测定结果进行了分析。本文尝试用高效液相色谱法对粪便胆汁酸进行分析。使用具有Zorbax C8柱的Shimazu仪器（RF-530）进样样品用于HPLC。分析单个胆汁酸中的胆酸、鹅去氧胆酸、去氧胆酸和石胆酸。但胆汁酸不能用HPLC分析。在本实验中，用气相色谱法分析粪便胆汁酸比用高效液相色谱法更好。

项目成果

牧野孝史: クリニカ. 14. 189-193 (1987)

牧野隆：临床。14。189-193（1987）

Takashi Makino: Tokai J.Clin.Med.9. 297-305 (1984)

牧野隆：东海 J.Clin.Med.9。

Takashi Makino: "Fecal Bile Acid Excretion in Patients with Colon Cancer" KLINIKA. 14. 189-193 (1986)

Takashi Makino：“结肠癌患者粪便胆汁酸排泄”KLINIKA。

Takashi,Makino: Tokai J.Clin.Med.9. 297-305 (1984)

Takashi，Makino：Tokai J.Clin.Med.9。

牧野孝史: 神奈川胆石症研究会誌. 6. 16-19 (1981)

牧野隆：神奈川胆石病研究会杂志 6. 16-19 (1981)。