Application of monoclonal antibody, NCC-ST-439 for serodiagnosis of carcinoma patients

单克隆抗体NCC-ST-439在癌症患者血清学诊断中的应用

基本信息

  • 批准号:
    61570658
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1986
  • 资助国家:
    日本
  • 起止时间:
    1986 至 1987
  • 项目状态:
    已结题

项目摘要

NCC-ST-439 is a monoclonal antibody raised against a gastric carcinoma xenograft. The antigen defined by NCC-ST-439 was found to be high molecular glycoprotein, had a molecular weight more than 5x10^6Da., with sialyl-sugar residue as its epitope. A sandwich radio-immunometric assay for this new tumor-associated carbohydrate antigen was developed in order to evaluate its usefulness as a tumor marker. More than 600 serum samples from normal donors and patients with various malignant and non-malignant disorders were assayed. The cut-off value was set at 12 units/ml. In normal donors, 97.0%(226/233) of sera had a negative antigen value except for 7 serum samples from young females. In patients with malignant disorders, 34.2%(82/240) had a positive antigen value, particularly 64.0% (16/25) of patients with pancreatic carcinoma, 66.7% (16/24) of patients with recurrent colorectal carcinoma and 54.5% (6/11) of patients with recurrent breast carcinoma. In patients with non-malignant disorders, 6.0% (7/116) had a positive antigen value, but this was lower than 20 units/ml.The positive rate in benign hepatobiliary disorders including gallstones, hepatitis and liver cirrhosis was especially low at 4.3%(1/23). Serial examinations of sera from patients with colorectal carcinoma revealed that antigen value changed according to disease status. We concluded that determination of serum NCC-ST-439 antigen in combination with CEA and/or CA19-9 would be useful for monitoring a wide variety of carcinomas.
NCC-ST-439是针对胃癌异种移植物产生的单克隆抗体。NCC-ST-439所定义的抗原为高分子糖蛋白,分子量大于5 × 10^6Da。以唾液酸糖残基为抗原表位。开发了这种新的肿瘤相关糖抗原的夹心放射免疫测定法,以评估其作为肿瘤标志物的有用性。对来自正常供体和患有各种恶性和非恶性疾病的患者的600多份血清样品进行了测定。临界值设定为12单位/ml。正常献血员血清中除7份年轻女性血清外,97.0%(226/233)的血清抗原阴性。在恶性肿瘤患者中,阳性率为34.2%(82/240),其中胰腺癌阳性率为64.0%(16/25),复发性结直肠癌阳性率为66.7%(16/24),复发性乳腺癌阳性率为54.5%(6/11)。在非恶性疾病患者中,6.0%(7/116)的患者抗原阳性,但低于20单位/ml,在良性肝胆疾病(包括胆结石、肝炎和肝硬化)中阳性率特别低,仅为4.3%(1/23)。大肠癌患者血清的系列检查显示,抗原值根据疾病状态而变化。我们得出结论,血清NCC-ST-439抗原与CEA和/或CA 19 -9的组合测定将有助于监测各种癌症。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kokichi Sugano: "Sandwich radioimmunometric assay for a new tumor-associated carbohydrate antigen difened by monoclonal antibody, NCC-ST-439" Jpn. J. Cancer Res.
Kokichi Sugano:“通过单克隆抗体 NCC-ST-439 区分的新型肿瘤相关糖抗原的三明治放射免疫测定法”Jpn。
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K.Sugano, M.Watanabe, S.Kodaitra, et al: "Application of new monoclonal antibody, NCC-ST-439 for serodiagnosis of carcinoma patients." Image Technology & Information Display. 18. 1111-1116 (1986)
K.Sugano、M.Watanabe、S.Kodaitra 等人:“新型单克隆抗体 NCC-ST-439 在癌症患者血清学诊断中的应用”。
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菅野康吉,渡辺昌彦,小平進ほか: 映像情報. 18. 1111-1116 (1986)
Yasukichi Kanno、Masahiko Watanabe、Susumu Kodaira 等:视频信息。18. 1111-1116 (1986)。
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菅野康吉 他: 映像情報メディカル. 18. 1111-1116 (1986)
Kokichi Kanno 等人:图像信息医学。18. 1111-1116 (1986)
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Kokichi Sugano: Proceeding of Kagoshima international symposium glycoconjugate in medicine Heterogeneity of carbohydrate chains of NCC-ST-439 antigen in sera of carcinoma patients defined by double determinant sandwich radioimmunometric assay, using. mono
Kokichi Sugano:鹿儿岛国际糖复合物医学研讨会论文集通过双行列式夹心放射免疫测定法确定癌症患者血清中 NCC-ST-439 抗原碳水化合物链的异质性。
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