Investigation of angiogenesis factor. - Basic research for a treatment of brain ischemic diseases including moyamoya disease. -

本研究的目的是从人脑肿瘤、脑血管畸形和/或炎症组织中鉴定一种血管生成因子，用于治疗包括烟雾病在内的脑缺血性疾病。作为第一步，对人脑肿瘤的手术标本进行了研究，在45KD处发现了一个异常点，但其功能尚未明确。下一步，对脑瘤培养液的上清液进行尽可能多的积累，同时对手术中不可避免地去除的人脑毛细血管内皮细胞进行分离。分离培养的细胞系后来被污染，商业上可获得的细胞系被用来检测细胞的增殖活性。作为最后一步，检测了脑膜瘤和已建立的人胶质瘤细胞A-172的培养上清。结果：从脑膜瘤组织匀浆中获得的单峰与对照组相比无明显差异，而从胶质瘤培养上清液中获得的单峰明显增强了细胞增殖能力。在这两种情况下，电泳法均显示多个峰。

The purpose of this investigatio was to identify an angiogenesis factor from human brain tumor, cerebrovascular malformation, and/or inflamatory tissue for the treatment of cerebral ischemic diseases including moyamoya disease. As a first step operative specimen of human brain tumor was investigaied and an abnormal spot was identified at 45KD, but of which function was not specified. As a next step the supernatant of the brain tumor cultured fluid was acumulated as much as possible and at the same time separation of the endothelial cell of the capillary blood vessels of the human brain inevitably removed during the operation was carrued out. This separated and cultured cellline was later contaminated and commercially availavle cell line was used to cheque the cell proliferative acrivity. As a last step meningioma and supernatant of established human glioma cell A-172 were examined. In each case a single peak was obrained using heparin affinity and mono-S colums.Results; A single peak obtained from the homogenate of meningioma did not show significant incerease in cell proliferative activity as compared with controls whereas that obtained from the supernatant of the glioma cultured fluid showed significant increase in ability to promote cell proliferation. In both case the electrophoresis demonstrated poly peaks.