Immunohistochemical Funicular Orientation of Peripheral Nerve

1.周围神经索状定位的免疫组化研究将免疫组化方法引入周围神经索状定位。我们用市售的抗体和染色试剂盒对大鼠和猪的脊神经根进行了染色。用抗胆碱乙酰转移酶抗体作为第一抗体，用此方法可区分大鼠脊神经前、后根。而在前、后根的组织化学方法比免疫组化方法更有优势.周围神经损伤后逆行性改变的组织学和组织化学研究结扎大鼠坐骨神经和臀肌分支，观察逆行性改变的组织学和组织化学变化，生化方法观察到中央断端乙酰胆碱酯酶活性降低，表现为乙酰胆碱酯酶阳性轴突横截面积减少。结果显示，结扎臀肌分支后，中央断端的轴突横截面积明显减少，有髓小纤维明显增多。电镜下乙酰胆碱酯酶活性的观察表明，在结扎后的大鼠坐骨神经和臀肌分支中，有髓轴突的活性随时间的推移而增强，分别出现在距结扎点15 mm和5 mm以上的区域。

1. Immunohistochemical funicular orientation of peripheral nerveImmunohistochemical method was introduced into the funicular orientation of peripheral nerve. We stained the spinal roots of rat and pig with commercialy obtainable antibodies and staining kit. An anti- choline acetyltransferase antibody was used as the first antibody.We could differentiate rat anterior and posterior spinal roots with this method. The histochemical method was, however, more advantageous than the immunohistochemical method in theanterior and posterior roots.2. Histological and histochemical study on the retrograde changes of injured peripheral nerveRat sciatic nerve and gluteal muscle branch were ligated and retrograde changes were observed histologically and histochemically.The decrease of acetylcholinesterase activity in the central stump, which has been observed by biochemical method, was observed as the decrease of teh cross-sectional area of the acetylcholinesterase positive axons. The decrease of the cross-sectional area of the axons in the central stump was confirmed statistically using micro-computer assisted image analyser.Small myelinated fibers have remarkably increased in number in the central stump of the ligated gluteal muscle branch. But electron microscopic observation of acetylcholinesterase activity revealed that only 10 % to 20 % of the small myelinated axons in the central stump showed the enzymatic activity.These retrograde changes progressed with the lapse of time after the ligationand were observed at the region more than 15 mm and more than 5 mm proximal to the ligated site in the ligated rat sciatic nerve and gluteal muscle branch respectively.