A serological study of tumour specific membrane antigens of cultured ovarian cancer cell lines

采用免疫黏附（IA）试验研究了培养的人卵巢癌细胞系对肿瘤相关抗原的体液抗体。对来自12名卵巢癌患者的173份血清进行了两种卵巢癌细胞系的检测；久保和l -久保。2例患者血清对l -久保细胞系呈IA阳性，4例患者血清经牛红细胞排除异源膜抗原抗体后，经这些细胞系自身的淋巴母细胞进一步吸收后，对久保细胞系呈IA阳性。经富含OFA的组织（胎儿脑）吸收后，血清样品无反应性。这些结果表明，乳腺癌患者的血清中含有OFA-I抗体，但在使用两种卵巢癌培养细胞系进行IA检测时，未检测到卵巢组织类型特异性抗原。

Humoral antibodies to tumour associated antigens of cultured human ovarian cancer cell lines were studied using the immune adherence (IA) test. One hendred seventy three sera from 12 ovarian cancer patients were tested ageinst two ovarian cancer cell lines ; Kubo and L-Kubo. Sera from 2 patients were IA positive to L-Kubo cell line and sera from 4 patientes to Kubo cell line after absorption with bovine erythrocytes to exclude antibody to heterologous membrane antigens and after further absorption with lymphoblastoid cells autologous to these cell lines.Aftr absorption with OFA rich tissue (fetal brain), no reactivity remained in the sera samples. These results indicate the sera of patients with breast cancer contain antibodies to OFA-I,but do not detect ovarian histologic type specific antigens as tested by IA using two ovarian cancer cultured cell lines.