Age-related alteration in adrenoceptor and its physiological responses.

肾上腺素受体的年龄相关变化及其生理反应。

• 批准号: 61571097
• 负责人: OHSHIKA Hideyo
• 金额: $ 1.28万
• 依托单位: Sapporo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61571097/
• 关键词: Aging; Adrenoceptor; Signal transduction; Cholesterol; phospholipid ratio; Membrane viscosity; Cerebral cortex; Parotid gland; 心室筋; 【α_1】-アドレナリン受容体; 〔【^3H】〕inositol phosphates; 【K^+】放出; ラット耳下腺

We investigated the relationship between the adrenoceptor signal transduction and the membrane composition in rat cerebral cortex, parotid gland and ventricular myocardium during aging. 1. Cerebral cortex: The density of specific [^3H]prazosin binding site reduced with advanced age, but the binding affinity for [^3H]prazosin did not. Norepinephrine (NE)-induced formation of [^3H]inositol phosphates increased with advanced age. The cholesterol content in membranes increased with advanced age; concomitantly, an increase of the ratio cholesterol/phspholipid was observed. The membrane viscosity as measured by using 1,6-diphenyl-1,3,5-hexatriene increased with advanced age.2. Parotid gland: NE-induced of K^+ release from the slices reduced with advanced age. The density of specific [^3H]prazosin bindng site increased with advanced age, but the binding affinity for [^3H]prazosin did not. NE-induced formation of [^3H]inositol phosphates decreased with advanced age. No difference in isoprotere … More nol (ISO)-induced decrease in K^+ release and increase in cyclic AMP content were observed at any age examined. But the binding affinity for [^3H]dihydroalprenolol binding site reduced with advanced age, and the binding affinity for [^3H]dihydroalprenolol increased. The cholesterol content in membranes decreased with advanced age; concomitantly, a decrease of the ratio cholesterol/phospholipid was observed. The membrane viscosity decreased with advanced age.3. Ventricular myocardium: The density of specific [^3H]prazosin binding site reduced with adanced age, and the binding affinity for [^3H]prazosin increased. NE-induced formation of [^3H]inositol phosphates decreased with advanced age. The cholsterol content decreased with advanced age; concomitantly, a decrease in the ratio cholesterol/phospholipid was observed. The membrane viscosity decreased with advanced age.These results suggest that the both altered cholesterol/phospholipid ratio and/or membrane viscosity during aging may account for the changes in adrenoceptor signal transduction. Less

本实验研究了大鼠大脑皮层、腮腺和心室肌细胞膜组成与肾上腺素受体信号转导的关系。1.大脑皮层：[^3H]哌唑嗪特异性结合位点的密度随年龄增长而降低，但与[^3H]哌唑嗪的结合亲和力则不随年龄增长而降低。随着年龄的增长，去甲肾上腺素（NE）诱导的[^3H]肌醇磷酸的形成增加。膜中的胆固醇含量随着年龄的增长而增加;同时，观察到胆固醇/磷脂的比率增加。用1，6-二苯基-1，3，5-己三烯测定的膜粘度随年龄的增长而增加.腮腺：NE诱导的脑片K^+释放随年龄增长而减少。[^3H]哌唑嗪特异性结合位点密度随年龄增长而增加，而结合亲和力则不随年龄增长而增加。NE诱导的[^3H]肌醇磷酸的形成随着年龄的增长而减少。异丙孕酮无差异 ...更多信息 在所观察的任何年龄，都观察到了nol（ISO）诱导的K^+释放减少和cAMP含量增加。但随着年龄的增长，[^3H]二氢烯丙洛尔结合位点的结合亲和力降低，而[^3H]二氢烯丙洛尔结合位点的结合亲和力增加。膜中的胆固醇含量随着年龄的增长而降低;同时，观察到胆固醇/磷脂比降低。膜粘度随年龄的增长而降低.心室心肌：[^3H]哌唑嗪特异性结合位点密度随年龄增长而降低，结合亲和力随年龄增长而增加。NE诱导的[^3H]肌醇磷酸的形成随着年龄的增长而减少。胆固醇含量随着年龄的增长而降低;同时，观察到胆固醇/磷脂比降低。细胞膜粘度随增龄而降低，提示衰老过程中胆固醇/磷脂比值和/或细胞膜粘度的改变可能是肾上腺素受体信号转导的变化。少

项目成果

A.Miyamoto: (J.Pharm.Pharmacol.).

A.Miyamoto：（J.Pharm.Pharmacol.）。

A. Miyamoto: "Age-related altereation in _1-adrenergic singnal transduction of ratparotid gland." Submitted to Neurochem. Res.

A. Miyamoto：“大鼠腮腺_1-肾上腺素能信号转导中与年龄相关的改变。”

T.Saito: Japan.J.Pharmacol.43. 73P (1987)

T.Saito：日本.J.Pharmacol.43。

A.Miyamoto: Japan.J.Pharmacol.43. 205P (1987)

A.Miyamoto：Japan.J.Pharmacol.43。

宮本篤: 日薬理誌. 90. 7S (1987)

宫本敦：日本药理学杂志 90. 7S (1987)